Rabbit anti-Human/Monkey CD4 Monoclonal Antibody for Flow Cytometry - P01730

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4, also known as T-cell surface antigen T4/Leu-3, is a T-cell surface glycoprotein encoded by the CD4 gene, belonging to the immunoglobulin superfamily.
• Functions as a coreceptor for MHC class II molecules in T-cells, interacting with the T-cell receptor (TCR) and MHC class II on antigen-presenting cells, and recruiting the Src kinase LCK to trigger intracellular signaling pathways leading to T-helper cell activation, lymphokine production, motility, and adhesion.
• Participates in T-helper cell development in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
• In macrophages and NK cells, CD4 mediates differentiation, activation, cytokine expression, and cell migration through a TCR/LCK-independent pathway.
• Localizes to the cell membrane as a single-pass type I membrane protein.
• Highly expressed in T-helper cells, and also present in other immune cells including macrophages, dendritic cells, and NK cells.
• Post-translational modifications include disulfide bonds, glycosylation, palmitoylation, and phosphorylation; structural features include immunoglobulin domains, a signal peptide, and a transmembrane helix.
• Serves as the primary receptor for HIV-1, mediating viral entry, and is down-regulated by HIV-1 Vpu; also acts as a receptor for Human Herpesvirus 7 (HHV-7). Related references: PMID:12089508, PMID:16331979, PMID:2214026, PMID:17346169, PMID:7909607

Experimental Guidance and Technical Tips

• The immunogen is a recombinant human CD4 protein, suggesting potential reactivity against both native and denatured forms; validation in the relevant sample system is recommended.
• This conjugate is designed for flow cytometry; optimal antibody titration should be determined for each cell type and experimental condition.
• As a surface marker for T-helper cells, CD4 staining should be combined with appropriate gating strategies (e.g., CD3+ lymphocytes) to accurately identify T-cell subsets.
• Cross-reactivity is reported for cynomolgus monkey; for other non-human primate species, verify reactivity experimentally.

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CamelBio connects diagnostic manufacturers and researchers to high-quality IVD raw materials like this anti-CD4 monoclonal antibody for flow cytometry, supporting immunology and infectious disease applications. Our one-stop platform offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, streamlining your development from concept to clinic.