Rabbit anti-Human/Monkey CD4 Monoclonal Antibody for FC - P01730

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4 is a 51 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, also known as T4 or Leu-3.
• It functions as a coreceptor for MHC class II molecules, binding simultaneously with the T-cell receptor (TCR) and MHC class II on antigen-presenting cells, leading to LCK recruitment and downstream signaling.
• CD4 is essential for T-helper cell development in the thymus and drives monocyte-to-macrophage differentiation in a TCR/LCK-independent manner.
• Highly expressed on T-helper cells, CD4 is also present on macrophages, dendritic cells, and NK cells, mediating adhesion and migration.
• CD4 is the primary receptor for HIV-1, facilitating viral entry into host cells (PubMed:12089508, PubMed:16331979, PubMed:2214026). Related references: PMID:12089508, PMID:16331979, PMID:2214026
• HIV-1 Vpu protein downregulates CD4 expression to evade immune responses (PubMed:17346169). Related references: PMID:17346169
• CD4 also acts as a receptor for Human Herpesvirus 7 (HHV-7) (PubMed:7909607). Related references: PMID:7909607
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, palmitoylation, and phosphorylation.

Experimental Guidance and Technical Tips

• This antibody targets the extracellular domain of CD4, making it suitable for live-cell flow cytometry without permeabilization.
• The fluorophore is excited by the 488 nm laser and emits in the green channel (typically FITC/GFP filter set); verify compatibility with your cytometer configuration.
• To achieve optimal staining, titrate the antibody to determine the appropriate concentration for your specific cell type and experimental conditions.
• Use appropriate isotype controls and fluorescence-minus-one (FMO) controls to establish gating strategies and assess background staining.
• CD4 expression varies among T-cell subsets and activation states; validate the antibody in your system using well-characterized positive and negative cell populations.
• Because CD4 is the HIV receptor, avoid using cell lines or primary cells harboring HIV that could interfere with antibody binding or downregulate CD4.
• If intracellular staining is required, test the antibody’s performance after fixation and permeabilization, as these steps may affect epitope recognition.
• The antibody is reported to cross-react with cynomolgus monkey CD4; for other species, perform a validation experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this anti-CD4 monoclonal antibody alongside validated antibody pairs, bulk reagents, and custom solutions. Supporting immunology and infectious disease research, we streamline procurement from early development through clinical application. Our portfolio covers rare target sourcing, recombinant proteins, and ancillary materials to accelerate your diagnostic assay pipeline.