Rabbit anti-Human/Monkey CD4 mAb for Flow Cytometry - P01730

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4 is a T-cell surface glycoprotein, also known as T4/Leu-3, belonging to the immunoglobulin superfamily. It functions primarily as a coreceptor for MHC class II molecules on antigen-presenting cells.
• In T-helper cells, CD4 interacts simultaneously with the T-cell receptor (TCR) and MHC class II:peptide complex, recruiting the Src kinase LCK to initiate signaling cascades that lead to T-cell activation, lymphokine production, and immune response.
• CD4 is highly expressed on the surface of T-helper cells, but is also present on other immune cells such as macrophages, dendritic cells, and NK cells, where it may modulate differentiation and cytokine expression.
• Subcellularly, CD4 is an integral membrane protein localized to the cell membrane, with a single transmembrane helix.
• CD4 serves as the primary receptor for human immunodeficiency virus-1 (HIV-1), facilitating viral entry into host cells. It is also a receptor for Human Herpesvirus 7 (HHV-7) (PubMed:12089508, PubMed:16331979, PubMed:2214026, PubMed:7909607). Related references: PMID:12089508 PMID:16331979 PMID:2214026 PMID:7909607
• HIV-1 Vpu protein downregulates CD4 expression to counteract immune response (PubMed:17346169). Related references: PMID:17346169
• Post-translational modifications include N-linked glycosylation, palmitoylation, and phosphorylation, which are important for protein stability and signaling.
• The protein contains a signal peptide, extracellular immunoglobulin-like domains, a transmembrane region, and a short cytoplasmic tail.

Experimental Guidance and Technical Tips

• This conjugate is designed for direct flow cytometry. is excited by the 405 nm violet laser and emits at 450 nm, compatible with standard violet channels.
• Titrate the antibody to determine the optimal working concentration for your cell type and staining conditions. Typically, a starting concentration of 0.1-1 µg per million cells is recommended.
• Include appropriate isotype controls and viability dyes to ensure accurate gating and compensation.
• For surface staining of human or cynomolgus PBMCs, incubate cells with the antibody at 2-8°C for 30 minutes in the dark, then wash before acquisition.
• CD4 is a well-established marker for T-helper cells, making this antibody suitable for immunophenotyping panels in immune monitoring, infectious disease, and vaccine research.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting manufacturers and laboratories with validated antibodies, antigens, and ancillary reagents. This CD4 monoclonal antibody exemplifies our offering in immune cell surface markers, ideal for flow cytometry applications in clinical diagnostics and translational research. We support custom conjugation, bulk orders, and rapid sourcing of rare targets to accelerate your assay development from concept to clinic.