Rabbit anti-Human/Monkey CD38 PolymAb® for FC - P28907

Core Product Specifications and Parameters

Biological Background: CD38 Function and Localization

• CD38 is a multifunctional transmembrane glycoprotein with ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase activities, generating second messengers cADPR and NAADP+ that regulate Ca²⁺ mobilization and insulin secretion. Related references: PMID:7961800 PMID:8253715 PMID:16690024
• It acts as a receptor for CD31 (PECAM-1) on endothelial cells, mediating lymphocyte adhesion, activation, and transendothelial migration. Related references: PMID:9551996
• CD38 is crucial for dendritic cell maturation, influencing CCL21-driven migration, survival, and Th1 polarization. Related references: PMID:16293598
• In lamina propria T cells, CD38-CD31 interaction triggers LCK/LAT signaling and cytokine release. Related references: PMID:11259373
• Subcellularly, CD38 is a type II transmembrane protein located on the cell surface.
• Highly expressed in pancreas, liver, kidney, brain, testis, ovary, placenta, and certain cancers (malignant lymphoma, neuroblastoma).
• Features disulfide bonds, glycosylation, and alternative splicing, with a mature molecular weight of approximately 34 kDa.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your cell type. Use appropriate isotype controls and perform live/dead cell exclusion to ensure reliable gating.
• Human peripheral blood mononuclear cells (PBMCs) or CD38-expressing cell lines (e.g., Raji, Daudi) are suitable positive controls. Confirm cross-reactivity in cynomolgus monkey samples if relevant.
• The immunogen encompasses the extracellular domain (aa 43–300), so the antibody is expected to recognize cell surface CD38. Consider fixation/permeabilization only if intracellular detection is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents. For CD38-targeted studies and assay development, we offer bulk supply and custom sourcing solutions to accelerate your transition from concept to clinic.