Rabbit anti-Human/Monkey CD3 Monoclonal Antibody for Flow Cytometry - P07766

Core Product Specifications and Parameters

Biological Background: CD3 Epsilon Chain Function and Localization

• CD3ε (T-cell surface glycoprotein CD3 epsilon chain, also known as T3E) is an essential subunit of the T-cell receptor (TCR)-CD3 complex, playing a critical role in adaptive immune responses. Related references: PMID:15294938 | PMID:15546002 | PMID:2470098
• Upon TCR engagement, CD3 chains (including CD3ε) transmit activation signals via phosphorylation of their cytoplasmic ITAM motifs by Src kinases LCK and FYN. Related references: PMID:2470098 | PMID:40592325
• Phosphorylated CD3ε ITAMs create docking sites for ZAP70 kinase, triggering downstream signaling cascades required for T-cell activation (By similarity).
• CD3ε is vital for proper T-cell development and thymocyte maturation (By similarity).
• It mediates internalization and down-regulation of surface TCR-CD3 complexes through endocytosis sequences in its cytosolic region, regulating T-cell sensitivity. Related references: PMID:10384095 | PMID:26507128
• Beyond its coreceptor function, CD3ε acts as a receptor for ITPRIPL1, inhibiting T-cell activation by promoting NCK1 interaction, which blocks ZAP70 binding and ERK-NFkB signaling. Related references: PMID:12110186 | PMID:38614099
• The CD3ε protein (23kDa) is a single-pass type I membrane protein with an extracellular immunoglobulin domain, a transmembrane helix, and a cytoplasmic tail harboring ITAM motifs. It is localized to the plasma membrane of T lymphocytes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (aa 1-126), suggesting the antibody targets surface CD3ε; confirm specificity using appropriate controls.
• As an conjugate, this antibody is optimized for direct flow cytometry; titrate to determine the optimal staining concentration for your sample type.
• Cross-reactivity with cynomolgus monkey is reported; validate on other species if needed.
• For best results, use fresh peripheral blood mononuclear cells (PBMCs) or lymphoid tissue suspensions; avoid fixation that may mask the epitope.

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CamelBio serves as a versatile procurement partner for IVD raw materials, supporting T-cell immunology research and diagnostic development. Our portfolio includes validated antibody pairs, optimized monoclonal antibodies (conjugated or unconjugated), bulk ancillary reagents, and rare target sourcing. We streamline your workflow from early-stage research to clinical application, offering tailored solutions to meet your project needs.