Rabbit anti-Human/Monkey CD3 Monoclonal Antibody for FC - P07766

Core Product Specifications and Parameters

Biological Background: CD3 epsilon chain Function and Localization

• CD3 epsilon chain (CD3E) is a component of the T-cell receptor (TCR)-CD3 complex on T-lymphocytes, playing a crucial role in adaptive immunity. Related references: PMID:15294938, PMID:15546002, PMID:2470098

• Upon TCR engagement by antigen-presenting cells, CD3E transmits signals across the cell membrane, with its cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) being phosphorylated by Src kinases LCK and FYN, leading to downstream signaling activation. Related references: PMID:2470098, PMID:40592325

• CD3E ITAM phosphorylation provides docking sites for ZAP70, which becomes phosphorylated and activated, driving T-cell activation; the chain also participates in T-cell development.

• CD3E also mediates internalization and down-regulation of TCR-CD3 complexes via endocytosis sequences in its cytosolic region, regulating T-cell response. Related references: PMID:10384095, PMID:26507128

• Beyond its TCR coreceptor role, CD3E serves as a receptor for ITPRIPL1, which inhibits T-cell activation by promoting interaction with NCK1. Related references: PMID:12110186, PMID:38614099

• Subcellular localization is the cell membrane.

• Contains an immunoglobulin domain and a transmembrane helix; classified as a phosphoprotein, receptor, and part of the reference proteome.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your cell population.
• The immunogen spans the N-terminal 126 amino acids, likely targeting the extracellular domain; confirm surface staining on live cells and include an isotype control.
• CD3E is expressed on T lymphocytes; use appropriate gating strategies and validate reactivity with human or cynomolgus PBMCs or T-cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents for T-cell immunology and diagnostic development. This anti-CD3 antibody supports flow cytometry-based applications; CamelBio can also assist with bulk orders, custom conjugation, and assay optimization to accelerate your research and clinical projects.