Rabbit Anti-Human/Monkey CD25 Monoclonal Antibody for Flow Cytometry - P01589

Core Product Specifications and Parameters

Biological Background: CD25 Function and Localization

• CD25 is the interleukin-2 receptor alpha subunit (also known as TAC antigen or p55), encoded by the IL2RA gene.
• It functions as a receptor for interleukin-2 (IL-2) and is critically involved in the regulation of immune tolerance.
• Through its role on regulatory T cells (TREGs), CD25 suppresses the activation and expansion of autoreactive T-cells, preventing autoimmunity.
• The protein is a single-pass type I membrane glycoprotein with extracellular sushi repeats and a transmembrane helix.
• It contains disulfide bonds and undergoes glycosylation, which contribute to its structural stability and ligand binding.
• Genetic variants in IL2RA are associated with diabetes mellitus and other autoimmune disorders, highlighting its role in disease.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant fusion protein covering the extracellular domain (aa 22-213), ensuring recognition of the native conformation on intact cells, making the antibody suitable for flow cytometry.
• Consider using FACS buffer with 0.5% BSA or 1% serum to reduce non-specific staining, and include isotype controls to set gates.
• Validate the antibody in the relevant species (human or cynomolgus) by comparing unstained and stained cells, as well as using a positive control such as activated T cells.
• For conjugates, protect from light and use appropriate laser/filter settings (e.g., 633-647 nm excitation, 660-668 nm emission).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to high-quality IVD raw materials for immunology and flow cytometry applications. Our portfolio includes optimized monoclonal antibodies, conjugated reagents, and ancillary raw materials for T-cell and cytokine research. For specialized targets like CD25, we offer flexible supply solutions to support your assay development and scale-up needs.