Rabbit anti-Human/Monkey CD25 Monoclonal Antibody for FC - P01589

Core Product Specifications and Parameters

Biological Background: CD25 (IL2RA) Function and Localization

• CD25, also known as interleukin-2 receptor subunit alpha, TAC antigen, and p55, is encoded by the IL2RA gene. Alternative names include IL2R, IMD41, TCGFR, and IDDM10, reflecting its roles in immune regulation and disease.
• Functions as the ligand-binding alpha subunit of the high-affinity interleukin-2 (IL-2) receptor, essential for IL-2-mediated signaling in lymphocytes.
• Plays a critical role in immune tolerance by sustaining regulatory T cell (TREG) activity, which suppresses the activation and expansion of autoreactive T-cells.
• Localized to the plasma membrane as a single-pass type I membrane protein, with an extracellular region containing sushi domains.
• Post-translational modifications include N-linked glycosylation and intrachain disulfide bonds, which stabilize its structure and facilitate ligand interaction.
• Genetic variants in IL2RA are associated with immunodeficiency 41 (IMD41) and susceptibility to insulin-dependent diabetes mellitus type 10 (IDDM10), underscoring its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 22–213), targeting an exposed region likely preserved in native CD25. Confirm reactivity in the relevant experimental system.
• For flow cytometry, titrate the antibody to determine optimal staining concentration using a known positive cell line, such as activated human peripheral blood mononuclear cells (PBMCs) or CD25-expressing cell lines.
• Include proper isotype controls and viability dyes to gate live cells, as CD25 can be upregulated upon activation or in certain pathological states.
• Cross-reactivity with cynomolgus monkey allows preclinical translational studies; however, validate target expression levels in the chosen model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive B2B partner for IVD raw-material sourcing, offering one-stop access to validated antibodies, reagents, and technical support from concept to clinic. This anti-CD25 antibody supports immunology and autoimmune disease research, complementing our portfolio of monoclonal antibodies and ancillary reagents. For bulk quantities, custom conjugation, or rare-target raw-material procurement, our supply chain ensures rapid delivery and quality consistency.