Rabbit anti-Human/Monkey CD20 Monoclonal Antibody for Flow Cytometry - P11836

Core Product Specifications and Parameters

Biological Background: CD20 Function and Localization

• CD20 is a B-lymphocyte-specific membrane protein, also known as B-lymphocyte surface antigen B1, Bp35, Leu-16, and is encoded by the MS4A1 gene.
• It functions as a store-operated calcium (SOC) channel component, regulating calcium influx after B-cell receptor activation, which is crucial for B-lymphocyte development, differentiation, and activation. Related references: PMID:12920111 PMID:3925015 PMID:7684739 PMID:18474602
• CD20 localizes to the cell membrane, consistent with its role in calcium signaling.
• Expression is restricted to B-cells, making it a valuable marker for B-cell lineage.
• Post-translational modifications include palmitoylation and phosphorylation; a disulfide bond is also present, contributing to its structural stability.
• Alternative splicing produces isoforms with molecular weights of approximately 14 kDa and 33 kDa, as reflected by the product's protein weight specification.

Experimental Guidance and Technical Tips

• The conjugate is designed for direct flow cytometry; a titration experiment is recommended to determine the optimal antibody concentration for your specific cell population.
• As the immunogen covers amino acids 100-200, ensure that your target epitope is present in all isoforms of interest; validation in the relevant sample system is advised.
• For best results, use appropriate isotype controls and include viability staining when analyzing primary B-cell samples.
• Consider testing the antibody on known CD20-positive and negative cell lines (e.g., Raji vs. Jurkat) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. This Rabbit anti-Human/Monkey CD20 monoclonal antibody supports B-cell targeted research and diagnostic applications, with a focus on flow cytometry-based detection. We are your bridge from concept to clinic, offering comprehensive supply solutions to accelerate your IVD development.