Rabbit anti-Human/Monkey CD20 Monoclonal Antibody for Flow Cytometry - P11836

Core Product Specifications and Parameters

Biological Background: CD20 Function and Localization

• CD20 is a B-lymphocyte-specific membrane protein critical for regulating calcium influx, essential for B-cell development, differentiation, and activation. Related references: PMID:12920111, PMID:3925015, PMID:7684739
• It functions as a store-operated calcium channel component that promotes calcium influx upon B-cell receptor (BCR) stimulation. Related references: PMID:12920111, PMID:18474602, PMID:7684739
• CD20 is encoded by the MS4A1 gene and belongs to the membrane-spanning 4-domains subfamily A. Alternative splicing produces isoforms with predicted molecular weights of approximately 14 kDa and 33 kDa.
• The protein is primarily localized to the cell membrane and is specifically expressed on B-lymphocytes, making it a classic B-cell lineage marker.
• Post-translational modifications include phosphorylation and palmitoylation, and the protein contains disulfide bonds, contributing to its structural integrity.
• CD20 is a clinically validated pharmaceutical target for B-cell depletion therapies in oncology and autoimmune diseases.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide corresponding to amino acids 100-200 of human CD20, which maps to an intracellular domain; therefore, fixation and permeabilization may be required for intracellular staining in flow cytometry.
• Titrate the antibody to determine the optimal working concentration for your specific cell type and experimental conditions. Include appropriate isotype and fluorescence-minus-one (FMO) controls.
• The antibody is validated for cross-reactivity with human and cynomolgus CD20. For other species, empirical testing is recommended.
• Protein weight discrepancies (14kDa/33kDa) likely reflect detection of multiple CD20 isoforms; confirm the expected molecular weight in your system using known B-cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated one-stop source for IVD raw materials, offering rapid access to validated antibody reagents like this anti-CD20 monoclonal antibody for diagnostic and research applications. Whether you need validated antibody pairs, bulk ancillary reagents, or customized sourcing for rare targets, our streamlined supply chain accelerates your assay development from concept to clinic.