Rabbit anti-Human/Monkey CD20 Monoclonal Antibody for FC - P11836

Core Product Specifications and Parameters

Biological Background: CD20 Function and Localization

• CD20 (B-lymphocyte antigen CD20) is a B-cell-specific transmembrane protein belonging to the membrane-spanning 4-domains subfamily A (MS4A), encoded by the MS4A1 gene. Alternative splicing may generate multiple isoforms.
• It functions as a store-operated calcium (SOC) channel component, regulating calcium influx upon B-cell receptor (BCR) activation, which is essential for B-cell development, differentiation, and activation.
  Related references:
  PMID:12920111
  PMID:3925015
  PMID:7684739
  PMID:18474602
• CD20 is localized to the cell membrane and is expressed specifically on B-lymphocytes, but not on precursor B-cells or plasma cells, making it a key marker for B-cell identification.
• Post-translational modifications include palmitoylation and phosphorylation, which may regulate its membrane association and activity. It also contains disulfide bonds and is a lipoprotein.
• CD20 is a well-established pharmaceutical target; monoclonal antibodies against CD20 (e.g., rituximab) are used in the treatment of B-cell malignancies and autoimmune diseases, highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 100-200 of human CD20, which likely encompasses the large extracellular loop between transmembrane domains 3 and 4. This suggests the antibody may recognize an extracellular epitope, making it suitable for flow cytometry on live, unfixed cells.
• For flow cytometry, use the antibody at an optimal dilution determined by titration on human or cynomolgus peripheral blood mononuclear cells (PBMCs) or CD20-expressing B-cell lines. Ensure proper Fc receptor blocking and include appropriate isotype controls.
• If the antibody is provided in a conjugated format (e.g., dye), it can be used directly for single-step staining. If unconjugated, a fluorophore-labeled anti-rabbit secondary antibody is required for detection.
• Consider multiplexing with other B-cell markers (e.g., CD19, CD21) to characterize B-cell subsets in immunophenotyping panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our anti-CD20 monoclonal antibody supports B-cell research and diagnostic assay development. With expertise in rare target sourcing and a streamlined supply chain from concept to clinic, CamelBio accelerates your project timelines.