Rabbit anti-Human/Monkey CD20 mAb for Flow Cytometry - P11836

Core Product Specifications and Parameters

Biological Background: CD20 Function and Localization

• CD20 (MS4A1) is a B-lymphocyte-specific membrane protein that regulates calcium influx necessary for B-cell development, differentiation, and activation, and functions as a store-operated calcium channel component after B-cell receptor stimulation. Related references: PMID:12920111, PMID:3925015, PMID:7684739, PMID:18474602
• CD20 belongs to the membrane-spanning 4A family (MS4A) and is expressed on the surface of B-cells from pre-B to mature stages, but not on plasma cells.
• The protein undergoes alternative splicing, producing isoforms of approximately 33 kDa (canonical full-length) and 14 kDa, consistent with the molecular weights observed in the provided specifications.
• CD20 is subject to post-translational modifications including phosphorylation and palmitoylation, which may influence its membrane localization and function.
• Subcellularly, CD20 is an integral component of the cell membrane, acting as a multi-pass transmembrane protein.
• As a pharmaceutical target, CD20 is the antigen for monoclonal antibodies used in treating B-cell malignancies and autoimmune disorders.

Experimental Guidance and Technical Tips

• The immunogen was raised against an intracellular peptide (aa 100–200 of human CD20), suggesting the antibody may recognize an epitope accessible in permeabilized cells; for surface staining, validate performance on non-permeabilized vs. permeabilized samples.
• For flow cytometry, perform titration experiments to determine the optimal working concentration for your specific cell type, and include appropriate isotype controls.
• Use B-cell lines (e.g., Raji, Daudi) or peripheral blood mononuclear cells (PBMCs) as positive controls, and plasma cells or T-cell lines as negative controls.
• Cross-reactivity with cynomolgus monkey CD20 has been confirmed; cross-reactivity with other species should be independently validated if required.

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