Rabbit anti-Human/Monkey CD16 Monoclonal Antibody for Flow Cytometry - P08637/O75015

Core Product Specifications and Parameters

Biological Background: CD16 Function and Localization

• Protein Identity: CD16a is the low affinity immunoglobulin gamma Fc region receptor III-A, encoded by FCGR3A (GeneID 2214), with a molecular weight of approximately 29 kDa (UniProt: P08637). Alternative names include CD16, CD16a antigen, and Fc-gamma RIII-alpha.
• Core Function in ADCC: CD16a binds clustered IgG immune complexes to mediate antibody-dependent cellular cytotoxicity (ADCC) on NK cells, triggering lysis of antibody-coated target cells without binding monomeric IgG.
  Related references: PMID:11711607 PMID:21768335 PMID:22023369
• NK Cell Activation: Following IgG engagement, CD16a transduces signals through ITAM-containing adapter proteins, leading to phosphatidylinositol 3-kinase activation and calcium flux, which drive cytokine production and degranulation.
  Related references: PMID:1825220 PMID:23024279 PMID:2532305
• NK Cell Homeostasis: CD16a regulates NK cell survival and proliferation by preventing apoptosis of NK cell progenitors, contributing to the maintenance of NK cell populations.
  Related references: PMID:29967280 PMID:9916693
• Anti-Tumor Activity: Therapeutic antibodies engage CD16a to mediate ADCC against tumor cells; afucosylated IgG antibodies exhibit enhanced binding to CD16a, improving opsonization and tumor cell clearance.
  Related references: PMID:27670158 PMID:34485821 PMID:28566370
• Subcellular Localization and Tissue Specificity: CD16a is primarily localized to the cell membrane of NK cells and a subset of monocytes, with a soluble form generated by shedding. Expressed at high levels in natural killer cells and detected in circulating monocytes.
  Related references: PMID:2526846
• Role in Dengue Pathogenesis: During secondary Dengue virus infection, afucosylated IgG1 antibodies form complexes that bind CD16a with high affinity, facilitating virus entry into myeloid cells via antibody-dependent enhancement (ADE).
• Post-Translational Modifications: CD16a is a glycoprotein with disulfide bonds and phosphorylation sites, which are critical for its structure and signaling functions.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 20–208 of human FCGR3A/FCGR3B (extracellular domain), ensuring detection of native CD16 epitopes exposed on the cell surface.
• Optimized for flow cytometry (FC) applications; the dye conjugate provides bright fluorescence with low background when using appropriate filter sets.
• For optimal staining, titrate the antibody in your relevant sample system and include suitable isotype controls to set gates and compensation.
• Cross-reactivity with cynomolgus monkey CD16 allows for translational studies in non-human primate models.
• Consider fixation and permeabilization protocols carefully, as some epitopes may be sensitive; validate intracellular detection if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated monoclonal antibodies, bulk ancillary reagents, and tailored procurement solutions. For CD16-targeted applications, we supply high-quality anti-CD16 antibodies including dye-conjugated formats to support immunophenotyping and ADCC research. Let CamelBio streamline your supply chain from concept to clinic.