Rabbit anti-Human/Monkey CD16 Monoclonal Antibody for FC - P08637 / O75015

Core Product Specifications and Parameters

Biological Background: CD16 (FCGR3A/FCGR3B) Function and Localization

• CD16 is a low-affinity IgG Fc receptor existing as two isoforms: FCGR3A (CD16a) and FCGR3B (CD16b). FCGR3A is a transmembrane glycoprotein that mediates antibody-dependent cellular cytotoxicity (ADCC), cytokine release, and NK cell activation upon binding to antigen-IgG complexes. Related references: PMID:11711607 PMID:21768335 PMID:22023369
• FCGR3A signaling involves ITAM phosphorylation, PI3K activation, and intracellular calcium flux, resulting in cell degranulation and pro-inflammatory cytokine production. Related references: PMID:1825220 PMID:23024279 PMID:2532305
• It promotes the generation and survival of memory-like adaptive NK cells and prevents NK progenitor apoptosis. Related references: PMID:24412922 PMID:25786175 PMID:29967280 PMID:9916693
• FCGR3A drives the antitumor activity of therapeutic antibodies, with enhanced ADCC observed for afucosylated IgG1. Related references: PMID:27670158 PMID:34485821 PMID:28566370
• During Dengue virus infection, FCGR3A may facilitate antibody-dependent enhancement (ADE) of viral entry.
• The receptor is primarily expressed on the cell membrane of NK cells and a subset of monocytes, with a secreted form generated by proteolytic shedding.
• It belongs to the immunoglobulin superfamily, contains Ig-like domains, and undergoes N-linked glycosylation essential for proper folding and IgG binding.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 20–208), making this antibody suitable for surface staining on live cells without permeabilization.
• As a direct conjugate, no secondary antibody is required; incorporate an appropriate isotype control and Fc receptor blocking step to confirm specificity.
• Reported cross-reactivity with cynomolgus monkey supports use in preclinical models, but validation in the target species and tissue is recommended.
• Titrate the antibody to determine the optimal staining concentration for your cell type and experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material solutions, from validated antibody pairs and conjugates to bulk ancillary reagents. Our portfolio includes ready-to-use reagents for immune checkpoint and immuno-oncology targets such as CD16, supporting the development of flow cytometry-based diagnostic kits and ADCC assays. With one-stop sourcing and technical support, CamelBio accelerates your transition from concept to clinic.