Rabbit anti-Human/Monkey CD16 mAb for FC - P08637/O75015

Core Product Specifications and Parameters

Biological Background: CD16 Function and Localization

• CD16A (FCGR3A) is a low-affinity receptor for the Fc fragment of IgG, optimally activated by clustered antigen-IgG complexes to trigger antibody-dependent cellular cytotoxicity (ADCC).
  Related references: PMID:11711607, PMID:21768335, PMID:22023369
• The receptor does not bind free monomeric IgG, preventing inappropriate immune activation in the absence of antigenic challenge.
  Related references: PMID:11711607, PMID:21768335, PMID:22023369
• On natural killer (NK) cells, CD16A mediates IgG effector functions, triggering cytokine production (e.g., IFNG) and degranulation to combat viral infections.
  Related references: PMID:24412922, PMID:25786175
• CD16A contributes to the generation of memory-like adaptive NK cells capable of efficient ADCC-mediated elimination of virus-infected cells.
  Related references: PMID:24412922, PMID:25786175
• The receptor regulates NK cell survival and proliferation by preventing apoptosis of NK cell progenitors.
  Related references: PMID:29967280, PMID:9916693
• Signaling through ITAM-containing adapters activates PI3K and elevates intracellular calcium, driving NK cell activation, degranulation, or pro-inflammatory cytokine production depending on receptor phosphorylation status.
  Related references: PMID:1825220, PMID:23024279, PMID:2532305
• CD16A costimulates NK cells and can trigger lysis of target cells independently of IgG binding.
  Related references: PMID:10318937, PMID:23006327
• The receptor mediates the antitumor activities of therapeutic antibodies, especially those with afucosylated Fc domains, by enhancing ADCC and opsonization.
  Related references: PMID:27670158, PMID:34485821, PMID:28566370
• CD16A is a transmembrane glycoprotein located on the cell membrane and can be secreted; it is expressed on NK cells and a subset of circulating monocytes.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 20-208) of both FCGR3A and FCGR3B, suggesting that the antibody may recognize a conserved epitope shared between these two CD16 isoforms. Consider verifying isoform specificity in your target cell population.
• For flow cytometry, titrate the conjugate to determine the optimal staining concentration using relevant cell lines or primary cells known to express CD16, such as NK cells or monocytes.
• Because CD16A is shed from the cell surface, include appropriate controls (e.g., isotype control, unstimulated vs. stimulated samples) and consider evaluating both surface and intracellular staining if secretion is a concern.
• Cross-reactivity with cynomolgus monkey has been reported, but validate the reactivity in your specific cynomolgus cell type or tissue of interest before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies targeting critical immune markers like CD16. Whether you need optimized antibody pairs, bulk ancillary reagents, or support in sourcing rare-target raw materials, our integrated supply chain and technical expertise help streamline your journey from concept to clinic.