Rabbit anti-Human/Monkey CD14 Monoclonal Antibody for Flow Cytometry - P08571

Core Product Specifications and Parameters

Biological Background: CD14 Function and Localization

• CD14, also known as Monocyte differentiation antigen CD14, My23 antigen, and myeloid cell-specific leucine-rich glycoprotein, is a 40 kDa GPI-anchored glycoprotein.
• Functions as a coreceptor for bacterial lipopolysaccharide (LPS), forming a complex with LBP to deliver LPS to the LY96/TLR4 complex, thereby activating innate immune responses. Related references: PMID:1698311 PMID:23264655 PMID:20133493 PMID:22265692
• Upon LPS binding, CD14 signals through MyD88, TIRAP, and TRAF6, leading to NF-kappa-B activation, cytokine secretion, and inflammatory response. Related references: PMID:8612135
• Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides; these clusters trigger signaling from the cell surface and are subsequently targeted to the Golgi in a lipid-raft dependent pathway. Related references: PMID:16880211
• Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-). Related references: PMID:23880187
• Localizes to the cell membrane, membrane rafts, and Golgi apparatus, and is also secreted.
• Detected on macrophages, strongly expressed on monocytes, weakly on granulocytes, and on most tissue macrophages.
• Possesses leucine-rich repeats, disulfide bonds, and a GPI-anchor; involved in immunity, inflammatory response, and innate immunity.

Experimental Guidance and Technical Tips

• The antibody is a rabbit monoclonal, recognizing both human and cynomolgus CD14, and is validated for flow cytometry.
• For optimal flow cytometry results, titrate the antibody to determine the ideal working concentration for your specific cell population and staining protocol.
• Always include appropriate isotype controls and viability dyes to ensure accurate gating and exclude dead cells.
• Validate antibody performance in the relevant sample system, as CD14 expression levels may vary between cell types and activation states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For CD14-related research and assay development, we offer reliable sourcing of anti-CD14 antibodies, recombinant proteins, and complementary reagents. Contact us for comprehensive procurement support from concept to clinic.