Rabbit anti-Human/Monkey CD14 Monoclonal Antibody for FC - P08571

Core Product Specifications and Parameters

Biological Background: CD14 Function and Localization

• Monocyte differentiation antigen CD14, also known as My23 antigen or myeloid cell-specific leucine-rich glycoprotein, is a GPI-anchored glycoprotein encoded by the CD14 gene.
• Functions as a coreceptor for bacterial lipopolysaccharide (LPS); in concert with LBP, binds monomeric LPS and delivers it to the LY96/TLR4 complex, triggering innate immune responses. Related references: PMID:1698311, PMID:23264655, PMID:20133493, PMID:22265692
• Acts as a coreceptor for TLR2:TLR6 and TLR2:TLR1 heterodimers in response to diacylated and triacylated lipopeptides, respectively, with signaling from the cell surface and subsequent trafficking to the Golgi via lipid rafts. Related references: PMID:16880211
• Mediates cytokine release induced by electronegative LDL (LDL(-)) through an alternative inflammatory pathway. Related references: PMID:23880187
• Subcellular localization includes the cell membrane, membrane rafts, Golgi apparatus, and a secreted soluble form.
• Highly expressed on the surface of monocytes, macrophages, and weakly on granulocytes; detected on tissue macrophages.
• Key structural features include an N-terminal signal peptide, multiple leucine-rich repeats, a GPI anchor attachment site, and disulfide bonds, essential for proper folding and function.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 20-352 of human CD14, covering the extracellular leucine-rich repeat domain. This design suggests specificity for the mature, surface‑exposed region and should not cross‑react with the signal peptide.
• Since the antibody is directly conjugated to , it is optimized for flow cytometry. Consider titrating the antibody to determine the optimal concentration for your cell type, and include appropriate isotype controls.
• For detection of CD14 on cynomolgus monkey cells, validate reactivity under your specific experimental conditions, as cross‑reactivity may vary with sample preparation.
• Soluble CD14 present in serum or culture supernatant may compete with membrane‑bound CD14; therefore, washing cells thoroughly before staining is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This CD14 antibody supports immunology and innate immunity research, particularly in flow cytometry applications. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents in bulk, and can assist with rare‑target raw‑material sourcing to streamline your assay development.