Rabbit anti-Human/Monkey CD14 Monoclonal Antibody for FC - P08571

Core Product Specifications and Parameters

Biological Background: CD14 Function and Localization

• CD14, also known as My23 antigen or myeloid cell-specific leucine-rich glycoprotein, is encoded by the CD14 gene and belongs to the leucine-rich repeat (LRR) family.
• Functions as a coreceptor for bacterial lipopolysaccharide (LPS), forming a complex with LBP to deliver LPS to the LY96/TLR4 complex, thereby mediating innate immune response.
  Related references: PMID:1698311, PMID:23264655, PMID:20133493, PMID:22265692
• Triggers intracellular signaling via MyD88, TIRAP, and TRAF6, leading to NF-kappa-B activation, cytokine secretion, and inflammatory response.
  Related references: PMID:8612135
• Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides; these clusters trigger signaling from the cell surface and are subsequently targeted to the Golgi in a lipid-raft dependent pathway.
  Related references: PMID:16880211
• Binds electronegative LDL (LDL(-)), mediating cytokine release induced by LDL(-).
  Related references: PMID:23880187
• Localizes to the cell membrane, membrane rafts, and Golgi apparatus; a secreted form also exists.
• Expressed strongly on monocytes, weakly on granulocytes, and on most tissue macrophages; detected on macrophages at protein level.
  Related references: PMID:1698311
• Contains disulfide bonds, glycosylation sites, and a GPI-anchor, classifying it as a lipoprotein; it possesses multiple leucine-rich repeats.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 20-352) of human CD14, which may be recognized by the antibody in its native conformation on live cells, making it suitable for flow cytometry applications.
• For flow cytometry (FC), consider using appropriate isotype controls and performing antibody titration to optimize staining resolution.
• CD14 is highly expressed on monocytes and macrophages; validate staining specificity in relevant sample systems such as human peripheral blood mononuclear cells (PBMCs).
• The antibody is conjugated to , a fluorescent dye; ensure compatibility with your instrument's violet laser and appropriate filter sets; avoid fluorophore overlap with other markers in multicolor panels.
• Cross-reactivity with cynomolgus monkey extends utility to non-human primate models; verify cross-reactivity with specific monkey tissues/cells in preliminary experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your diagnostic development. This -conjugated anti-CD14 antibody targets a key innate immunity receptor, and CamelBio can assist in securing consistent supply, from research-grade batches to large-scale production, while also offering rare target raw material sourcing and technical consulting. Whether you need ready-to-use detection antibodies, complementary antigens, or custom conjugation services, our streamlined supply chain accelerates your project from concept to clinic.