Rabbit anti-Human/Monkey CD14 mAb for FC - P08571

Core Product Specifications and Parameters

Biological Background: CD14 Function and Localization

• CD14 (monocyte differentiation antigen CD14, My23) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein belonging to the leucine-rich repeat (LRR) superfamily.
• It acts as a coreceptor for bacterial lipopolysaccharide (LPS), binding monomeric LPS in concert with LBP and delivering it to the TLR4/MD-2 complex to trigger innate immune activation. Related references: PMID:1698311, PMID:20133493, PMID:22265692, PMID:23264655
• Downstream signaling is mediated by MyD88, TIRAP and TRAF6, leading to NF-κB activation and proinflammatory cytokine secretion. Related references: PMID:8612135
• CD14 also serves as a coreceptor for TLR2/TLR1 and TLR2/TLR6 heterodimers, recognizing triacylated and diacylated lipopeptides, respectively, and signaling from the cell surface followed by targeting to the Golgi in a lipid raft-dependent pathway. Related references: PMID:16880211
• It binds electronegative LDL (LDL(-)) and mediates cytokine release induced by modified LDL, connecting lipid metabolism to inflammatory responses. Related references: PMID:23880187
• CD14 is predominantly localized on the cell membrane, enriched in membrane rafts, and also detected in the Golgi; a soluble form is secreted.
• It is strongly expressed on monocytes and tissue macrophages and weakly on granulocytes, serving as a key surface marker for monocyte/macrophage identification.
• The protein contains intrachain disulfide bonds and is post-translationally modified by N-glycosylation, contributing to its structural stability and ligand interactions.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and staining conditions.
• Include appropriate isotype controls and, if possible, fluorescence minus one (FMO) controls to assess background staining.
• This antibody detects CD14 on human and cynomolgus peripheral blood mononuclear cells; confirm reactivity in your target sample.
• The immunogen (aa 20-352) covers the extracellular domain of CD14, suggesting potential recognition of both GPI-anchored membrane and soluble forms.
• Ensure that fixation/permeabilization protocols are compatible with the fluorophore if intracellular staining is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive one-stop sourcing solution for IVD raw materials, from early-stage development to clinical supply. For CD14-related innate immunity and inflammation research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw material sourcing to support your assay development and diagnostic kit production.