Rabbit anti-Human/Monkey CD127/IL-7Rα Monoclonal Antibody for Flow Cytometry - P16871

Core Product Specifications and Parameters

Biological Background: CD127/IL-7Rα Function and Localization

• Interleukin-7 receptor subunit alpha (CD127) is a type I transmembrane protein encoded by the IL7R gene. It serves as the primary receptor for interleukin-7 (IL-7), a cytokine essential for lymphocyte development, survival, and homeostasis.
• IL-7Rα also functions as a receptor for thymic stromal lymphopoietin (TSLP), a cytokine involved in allergic inflammation and T cell responses.
• Alternative splicing of the IL7R gene generates both membrane-bound and soluble isoforms (sIL-7R), the latter lacking the transmembrane region and being secreted into the extracellular milieu.
• Subcellularly, CD127 is predominantly localized to the cell membrane, while its soluble form is found in the secreted compartment and circulating blood.
• The receptor undergoes post-translational modifications including glycosylation and phosphorylation, which are critical for its binding affinity and signal transduction.
• Loss-of-function mutations in IL7R are associated with severe combined immunodeficiency (SCID), highlighting its non-redundant role in immune system development.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 21–236 of the human CD127 extracellular domain. This region is shared between membrane-bound and soluble isoforms, so the antibody may recognize both forms.
• For flow cytometry, consider validating the antibody on appropriate positive and negative cell lines or primary cells expressing CD127, such as T cells, and include isotype controls.
• Optimize staining conditions (e.g., antibody concentration, incubation time, and temperature) for your specific experimental system to ensure reproducible results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, providing a streamlined supply chain from antibody discovery to diagnostic application. This CD127/IL-7Rα monoclonal antibody supports research in immunology, hematology, and oncology, where IL-7 signaling is pivotal. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to meet your diagnostic development needs.