Rabbit anti-Human/Monkey CD127/IL-7Rα mAb - P16871

Core Product Specifications and Parameters

Biological Background: CD127/IL-7Rα Function and Localization

• Interleukin-7 receptor subunit alpha (IL-7Rα), also known as CD127, is a type I transmembrane glycoprotein encoded by the IL7R gene.
• It serves as the high-affinity receptor for interleukin-7 (IL-7) and also acts as a receptor for thymic stromal lymphopoietin (TSLP), forming heterodimers with the common gamma chain (γc) for IL-7 signaling and with TSLP receptor (TSLPR) for TSLP signaling.
• IL-7Rα plays a critical role in lymphocyte development, survival, and homeostasis; mutations in IL7R are associated with severe combined immunodeficiency (SCID).
• The receptor localizes primarily to the cell membrane, but a soluble secreted form (sIL-7Rα) is produced by alternative splicing and regulates IL-7 bioavailability.
• Post-translational modifications include N-glycosylation, disulfide bond formation, phosphorylation, and ubiquitination, which modulate receptor expression and signaling.
• The protein has a predicted molecular weight of approximately 51.6 kDa (calculated from amino acid sequence) and runs at around 52 kDa under reducing conditions due to glycosylation.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 21–236 of the extracellular domain, which encompasses the IL-7 binding region; this design likely yields an antibody capable of blocking ligand-receptor interaction—consider testing its neutralizing potential in functional assays.
• This conjugate is validated for flow cytometry (FC) on human samples; for cynomolgus monkey cross-reactivity, verify reactivity in relevant cell lines or PBMCs before inclusion in critical studies.
• When planning multi-color FC panels, assess spectral compatibility of with other fluorophores and titrate the antibody to optimize signal-to-noise ratio for your specific cell type.
• Due to the reported secreted form, intracellular staining protocols may need optimization to distinguish surface vs. intracellular receptor pools.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive range of IVD raw materials and technical support, streamlining the path from concept to clinic. This anti-CD127/IL-7Rα monoclonal antibody supports immunology and immune-oncology research, and we offer validated antibody pairs, optimized monoclonal/polyclonal antibody reagents, bulk ancillary supplies, and custom sourcing for rare targets. Whether you require conjugation flexibility or industrial-scale antibody production, CamelBio is your reliable procurement partner for high-quality raw materials.