Rabbit anti-Human/Monkey CD127/IL-7Rα mAb for FC - P16871

Core Product Specifications and Parameters

Biological Background: CD127/IL-7Rα Function and Localization

• Interleukin-7 receptor subunit alpha (CD127/CDw127) is a type I transmembrane glycoprotein encoded by the IL7R gene.
• It serves as the receptor for interleukin-7 (IL-7), and also binds thymic stromal lymphopoietin (TSLP), playing critical roles in lymphocyte development and homeostasis.
• CD127 belongs to the type I cytokine receptor family and forms heterodimers with the common gamma chain (γc) for IL-7 signaling, and with TSLP receptor for TSLP signaling.
• The protein is primarily localized at the cell membrane, but a secreted isoform exists through alternative splicing.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, phosphorylation, and ubiquitination.
• Mutations in IL7R are associated with severe combined immunodeficiency (SCID) and other immunological disorders.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 21-236) encompasses the extracellular domain; the resulting antibody is likely to recognize native surface-expressed CD127/IL-7Rα.
• For flow cytometry, consider titrating the antibody on your target cell population to determine the optimal staining concentration.
• Verify cross-reactivity in your specific experimental model; reactivity is confirmed for human and cynomolgus monkey.
• Use appropriate isotype controls and Fc receptor blocking to reduce nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to high-quality IVD raw materials, including this anti-CD127/IL-7Rα monoclonal antibody targeting the interleukin-7 receptor. Our one-stop sourcing platform offers validated antibody pairs, bulk ancillary reagents, and customized procurement solutions for cytokine receptors and other critical biomarkers. Partner with CamelBio to streamline your raw material supply from discovery to clinical application.