Rabbit anti-Human VCAM-1/CD106 Monoclonal Antibody for FC - P19320

Core Product Specifications and Parameters

Biological Background: VCAM-1/CD106 Function and Localization

• VCAM-1 (Vascular cell adhesion protein 1), also known as CD106 or INCAM-100, is a cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells. It plays an essential role in immune surveillance and inflammation. Related references: PMID:31310649
• As a major regulator of leukocyte adhesion to the endothelium, VCAM-1 binds to integrins on leukocytes, facilitating their recruitment to sites of inflammation. Related references: PMID:10209034
• During inflammatory responses, VCAM-1 engagement triggers the activation of calcium channels and the small GTPase RAC1, promoting leukocyte transendothelial migration. Related references: PMID:22970700
• VCAM-1 also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' signal in the context of MHC class-I presentation. Related references: PMID:35210567
• The VCAM1 gene undergoes alternative splicing, yielding multiple protein isoforms. The product detects isoforms with approximate molecular weights of 71 kDa, 74 kDa, and 81 kDa, consistent with the UniProt-calculated mass of 81,276 Da.
• Subcellular localization: Primarily found at the cell membrane; a secreted form is also produced.
• Tissue distribution: Highly expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissues.
• Post-translational modifications include glycosylation, disulfide bond formation, and ubiquitin conjugation (as indicated by UniProt keywords), which may influence its adhesive functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 25–698, encompassing the entire extracellular domain of human VCAM-1. This design favors recognition of the native, surface-exposed protein and is well-suited for flow cytometry (FC) applications.
• For FC detection of cell-surface VCAM-1, consider using activated human endothelial cells (e.g., HUVECs treated with TNF-α) or leukocyte cell lines known to bind VCAM-1. Validate staining in the relevant sample system.
• Because multiple splice variants exist, confirm that the antibody recognizes the isoform(s) of interest under your experimental conditions.
• Use appropriate isotype controls and Fc-receptor blocking steps to minimize non-specific binding in FC assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, supporting diagnostic manufacturers from concept to clinic. This anti-human VCAM-1/CD106 monoclonal antibody is part of our comprehensive portfolio for inflammation and immune-oncology research. Whether you need validated antibody pairs, rare-target antigens, or bulk ancillary reagents, CamelBio delivers optimized materials with expert technical support.