Rabbit anti-Human VCAM-1/CD106 mAb for FC - P19320

Core Product Specifications and Parameters

Biological Background: VCAM-1/CD106 Function and Localization

• Vascular cell adhesion protein 1 (VCAM-1), also known as CD106 or INCAM-100, is a cell adhesion glycoprotein encoded by the VCAM1 gene.
• Plays a pivotal role in immune surveillance and inflammation by mediating leukocyte adhesion to the endothelium. Related references: PMID:31310649
• Interacts with integrins on leukocytes to promote firm adhesion, a critical step in the inflammatory response. Related references: PMID:10209034
• Triggers calcium channel activation and RAC1 GTPase signaling during leukocyte transendothelial migration. Related references: PMID:22970700
• Functions as a quality‑control checkpoint in the bone marrow by providing a "don't‑eat‑me" signal within MHC class‑I presentation. Related references: PMID:35210567
• Localizes to the cell membrane and is also secreted; it is a single‑pass type I membrane protein.
• Predominantly expressed on inflamed vascular endothelial cells, macrophage‑like cells, and dendritic cells in both normal and inflamed tissues.
• Contains multiple immunoglobulin‑like domains, N‑glycosylation sites, and disulfide bonds; alternative splicing produces isoforms of varying molecular weights.

Experimental Guidance and Technical Tips

• The immunogen covers the entire extracellular domain (aa 25–698), indicating the antibody recognizes native VCAM-1 conformation; this supports its use in flow cytometry (FC) applications.
• As a directly conjugated antibody, no secondary detection step is required, simplifying the FC workflow.
• For optimal results, we recommend titrating the antibody to determine the ideal concentration for your specific cell type and experimental conditions.
• Include appropriate isotype control antibodies to assess non‑specific binding and ensure signal specificity.
• The reported protein weight of 71‑81 kDa reflects glycosylation‑dependent isoforms; if adapting for western blot, validate with appropriate lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one‑stop supplier of IVD raw materials, offering validated monoclonal antibodies like this anti‑VCAM‑1 product to support your diagnostic and research programs. Our procurement capabilities extend to optimized antibody pairs, bulk ancillary reagents, and sourcing of rare‑target biomaterials—streamlining your workflow from concept to clinic. For projects focused on inflammatory markers or endothelial biology, trust CamelBio to connect you with the raw materials and expertise you need.