Rabbit anti-Human β2 Microglobulin Monoclonal Antibody for Flow Cytometry - P61769

Core Product Specifications and Parameters

Biological Background: β2-Microglobulin Function and Localization

• Component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
• Exogenously applied M.tuberculosis EsxA or EsxA-EsxB can bind B2M and decrease its export to the cell surface, potentially leading to defects in class I antigen presentation. Related references: PMID:25356553
• Secreted and present on the cell surface, consistent with its role in immune surveillance.
• Contains an immunoglobulin-like domain and a disulfide bond; classified as a glycoprotein.
• Post-translational modifications include glycation and the formation of pyrrolidone carboxylic acid at the N-terminus.
• Disease-associated variants are linked to amyloidosis, reflecting its potential to form amyloid fibrils.
• The mature protein has a molecular weight of approximately 13.7 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 21–119 of human β2-Microglobulin, covering the mature secreted form; this design supports recognition of the native protein.
• As an conjugate, this antibody is ready for direct flow cytometry; titrate to determine the optimal concentration for your specific cell type and experimental conditions.
• Include appropriate isotype controls and consider Fc receptor blocking when staining Fc receptor-expressing cells.
• Validate performance in the relevant sample system, as cross-reactivity with other species is not expected based on the human-specific immunogen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with reliable IVD raw-material supply, including validated monoclonal antibodies like this anti-β2-Microglobulin clone. We offer comprehensive sourcing for immune-related targets—from bulk ancillary reagents to rare specificities—streamlining your development from concept to clinic with expert technical and logistical support.