Rabbit anti-Human β2 Microglobulin Monoclonal Antibody for Flow Cytometry - P61769

Core Product Specifications and Parameters

Biological Background: β2 Microglobulin Function and Localization

• Beta-2-microglobulin (B2M) is the light chain of the major histocompatibility complex (MHC) class I molecule and is essential for the presentation of peptide antigens to the immune system.
• It localizes to the cell surface as a component of MHC class I and is also secreted into extracellular fluids.
• The protein contains an immunoglobulin-like C1-set domain and a conserved disulfide bond that stabilize its structure.
• B2M is implicated in amyloidosis; certain mutations lead to the formation of amyloid fibrils, causing systemic amyloidosis.
• Mycobacterium tuberculosis evades immune recognition by secreting EsxA, which binds B2M and reduces its surface export, impairing antigen presentation. Related references: PMID:25356553
• Post-translational modifications include signal peptide cleavage, glycation, pyrrolidone carboxylic acid formation, and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature extracellular region (aa 21–119) of human B2M, enabling detection of the native protein on live cells by flow cytometry.
• This antibody is conjugated with , a far-red fluorophore; consider using appropriate isotype controls and optimizing the staining concentration for your specific cell type.
• Validate the antibody in your experimental system, as B2M expression can vary with cell activation, differentiation, or disease state.
• Cross-reactivity with other species has not been tested; verify if working with non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials and support, assisting diagnostic manufacturers and researchers from early development to clinical application. This anti-β2 Microglobulin monoclonal antibody exemplifies our offering of validated immunological reagents for flow cytometry and other platforms. Beyond this product, we supply optimized antibody pairs, bulk ancillary reagents, and can source rare-target raw materials to accelerate your assay development.