Rabbit anti-Human β2 Microglobulin mAb - P61769

Core Product Specifications and Parameters

Biological Background: B2M Function and Localization

• Beta-2-microglobulin (B2M) is the invariant light chain of the major histocompatibility complex class I (MHC I) molecule, essential for presenting intracellular peptide antigens to CD8+ T cells.
• It forms a non-covalent heterodimer with the MHC I heavy chain, stabilizing the complex and facilitating immune surveillance.
• B2M is secreted and expressed on the cell surface of nearly all nucleated cells, playing a critical role in adaptive immunity.
• The protein features an immunoglobulin-like domain, a disulfide bond essential for its structure, and undergoes N-linked glycosylation.
• Mutations in B2M are associated with hereditary systemic amyloidosis and various immune-related disorders.
• Exogenous Mycobacterium tuberculosis EsxA or EsxA-EsxB proteins bind to B2M, reducing its cell surface export and potentially impairing MHC I antigen presentation. Related references: PMID:25356553

Experimental Guidance and Technical Tips

• For flow cytometry, validate the antibody in your cell system using appropriate positive (e.g., peripheral blood mononuclear cells) and negative (B2M-deficient cells) controls.
• Surface staining can be performed on live cells without permeabilization, as B2M is displayed on the cell surface via MHC I.
• Protect the fluorophore-conjugated antibody from light; titrate to optimize signal-to-noise ratio.
• Consider that B2M is a small protein (13 kDa) and abundant in serum; pre-incubation steps should account for potential soluble B2M interference in some sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. We support every stage from concept to clinic, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our expertise in immune system targets like B2M enables efficient sourcing for MHC class I-related assay development. Contact our team for customized solutions and rare target procurement.