Rabbit anti-Human β2 Microglobulin mAb for Flow Cytometry - P61769

Core Product Specifications and Parameters

Biological Background: β2 Microglobulin Function and Localization

• Beta-2-microglobulin (B2M) is a 13 kDa non-glycosylated protein that serves as the invariant light chain of MHC class I molecules, essential for antigen presentation to cytotoxic T cells.
• As a member of the immunoglobulin superfamily, B2M contains an immunoglobulin-like domain and is non-covalently associated with the alpha chain of MHC I on the cell surface.
• B2M is synthesized as a pre-protein, processed into the mature form upon cleavage of the signal peptide, and is constitutively expressed in various tissues, reflecting its fundamental role in immune surveillance.
• Subcellularly, B2M is predominantly located on the cell surface as part of the MHC I complex and is also found in secreted form in body fluids.
• The protein undergoes post-translational modifications including glycation, disulfide bond formation, and pyrrolidone carboxylic acid formation at the N-terminus, which are important for its stability and function.
• Exogenously applied M. tuberculosis EsxA or EsxA-EsxB binds B2M and impairs its export to the cell surface, potentially compromising class I antigen presentation, which may represent an immune evasion mechanism. Related references: PMID:25356553
• Mutations in the B2M gene have been linked to amyloidosis and other diseases, highlighting its clinical significance in protein aggregation disorders.

Experimental Guidance and Technical Tips

• The antibody is generated against a recombinant immunogen spanning amino acids 21-119 of human B2M, encompassing the mature extracellular domain; thus, it likely recognizes the native conformation on live cells, making it suitable for flow cytometry.
• For flow cytometric analysis of cell surface β2-microglobulin, consider using this conjugate directly diluted in appropriate buffer; a standard staining protocol for human peripheral blood mononuclear cells or lymphocyte cell lines may be used.
• Validate the optimal antibody concentration by titration in the relevant experimental system, and include appropriate isotype controls to ensure specificity.
• As B2M is a soluble factor, supernatants or plasma may be analyzed using bead-based flow assays; however, the performance of this antibody in such assays has not been specifically tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, conjugated monoclonal antibodies, and bulk ancillary reagents. This β2-microglobulin antibody supports immunological studies and in vitro diagnostics targeting MHC class I, offering reliable detection in flow cytometry applications. For rare target sourcing, technical consultation, or scaling from concept to clinic, CamelBio provides integrated solutions to streamline your development workflow.