Rabbit anti-Human β2 Microglobulin mAb for Flow Cytometry - P61769

Core Product Specifications and Parameters

Biological Background: β2-Microglobulin Function and Localization

• Beta-2-microglobulin (B2M) is a low-molecular-weight protein (13.7 kDa) that forms the invariant light chain of the class I major histocompatibility complex (MHC I).
• It is essential for the stable cell surface expression of MHC I molecules and the presentation of peptide antigens to CD8+ T cells, playing a critical role in adaptive immune surveillance.
• B2M is secreted and also present on the cell surface as part of the MHC I complex; its secretion and surface expression can be modulated under pathological conditions.
• Exogenous Mycobacterium tuberculosis EsxA (or EsxA-EsxB) binds B2M and reduces its export to the cell surface without altering total protein levels, potentially impairing antigen presentation (PubMed:25356553). Related references: PMID:25356553
• The protein contains an immunoglobulin-like domain and a conserved disulfide bond; it is subject to various post-translational modifications including glycation, signal peptide cleavage, and pyrrolidone carboxylic acid formation.
• Keywords related to B2M include amyloid and amyloidosis, highlighting its involvement in dialysis-related amyloidosis where it aggregates into amyloid fibrils, and it has been identified as a disease variant in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature B2M extracellular domain (amino acids 21-119), suggesting the antibody targets the native conformation of surface β2-microglobulin. This design is well-suited for flow cytometry detection of cell surface MHC I complexes.
• For flow cytometry, users should titrate the antibody to determine the optimal concentration for their specific cell type and experimental conditions. Include appropriate isotype controls and Fc receptor blocking steps.
• While validated for flow cytometry, the antibody may be compatible with other applications such as immunohistochemistry or Western blotting after appropriate validation. Note that the conjugate () is optimized for flow cytometry and may not perform identically in other detection platforms.
• Consider cross-reactivity with non-human species if required; the antibody is tested on human samples, and homology across species might allow broader reactivity, but this must be experimentally confirmed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material procurement, offering a comprehensive portfolio from validated antibody pairs to bulk ancillary reagents. For immune monitoring and MHC class I analysis, we supply high-quality monoclonal antibodies like this anti-β2-microglobulin reagent, alongside customized sourcing of rare targets and technical consultation to bridge the gap from concept to clinic. Our efficient supply chain ensures researchers and diagnostic manufacturers receive consistent, reliable materials for their assay development needs.