Rabbit anti-Human ULBP2 Monoclonal Antibody for FC - Q9BZM5

Core Product Specifications and Parameters

Biological Background: ULBP2 Function and Localization

• ULBP2 (UL16-binding protein 2), also known as ALCAN-alpha, N2DL2, NKG2DL2, or RAET1H, is a GPI-anchored membrane protein and a member of the RAET1 family of NKG2D ligands.
• It serves as a ligand for the KLRK1/NKG2D receptor, an activating receptor expressed on natural killer (NK) cells and some T cell subsets, triggering cytotoxic immune responses.
• Upon engagement with NKG2D, ULBP2 mediates NK cell-mediated cytotoxicity against target cells, playing a critical role in tumor immunosurveillance.
• ULBP2 is localized on the cell membrane via a GPI anchor, but a secreted form also exists; it is additionally detected in the endoplasmic reticulum.
• Its expression is highly tissue-restricted: it is found in various cancer cell lines and fetal tissues, but is absent in normal adult tissues, highlighting its potential as a tumor-associated antigen.
• The protein features a signal peptide, N-glycosylation sites, and disulfide bonds, which are important for its proper folding and function.
• ULBP2 is known to interact with viral immune evasion proteins (e.g., human cytomegalovirus UL16), hence its original identification as a UL16-binding protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 26–217) of human ULBP2, encompassing the NKG2D-binding region; this design supports detection of surface-exposed ULBP2 in live-cell flow cytometry.
• For flow cytometric analysis, stain viable cells without permeabilization to assess membrane-localized ULBP2, as permeabilization may lead to intracellular staining of the ER-associated fraction.
• Include appropriate positive controls (e.g., ULBP2-expressing cancer cell lines) and negative controls (normal tissue cells) to validate specificity, in line with the protein's restricted expression profile.
• Titrate the antibody to determine the optimal concentration for your experimental system, and ensure proper compensation when multiplexing with other fluorophores.
• Due to potential shedding or secretion of ULBP2, carefully choose staining conditions (time, temperature) to minimize loss of surface antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined one-stop platform for procuring IVD raw materials, from validated antibody pairs to bulk ancillary reagents. Our portfolio includes high-quality monoclonal antibodies targeting immuno-oncology markers like the ULBP2/NKG2D axis, supporting assay development for NK cell biology and cancer research. With expertise in rare target sourcing and technical consulting, CamelBio helps accelerate your project from concept to clinic.