Rabbit anti-Human TROP-2 Monoclonal Antibody for IF/ICC, FC - P09758

Core Product Specifications and Parameters

Biological Background: TROP-2 Function and Localization

• Protein Identity and Synonyms: TROP-2 (Tumor-associated calcium signal transducer 2), encoded by the TACSTD2 gene, is also known as cell surface glycoprotein Trop-2, membrane component chromosome 1 surface marker 1 (M1S1), pancreatic carcinoma marker protein GA733-1, and epidermal glycoprotein 1 (EGP1).
• Growth Factor Receptor Function: TROP-2 may function as a growth factor receptor, potentially mediating intracellular calcium signals and playing a role in cell proliferation and survival.
• Subcellular Localization: The protein is localized to the membrane, consistent with its role as a transmembrane receptor. It contains an extracellular domain, a single transmembrane helix, and a short cytoplasmic tail.
• Tissue Expression: TROP-2 is expressed in placenta and pancreatic carcinoma cell lines, highlighting its relevance in developmental biology and oncology.
• Tumor Antigen and Clinical Relevance: Recognized as a tumor antigen, TROP-2 is overexpressed in various epithelial cancers and has emerged as a diagnostic marker and therapeutic target, particularly in antibody-drug conjugate development.
• Post-translational Modifications: The protein is characterized as a glycoprotein with disulfide bonds, which are critical for its structural stability and cell surface expression.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 27–274, which corresponds to the extracellular domain of TROP-2. The resulting antibody likely recognizes an extracellular epitope and is suitable for live-cell staining in flow cytometry (FC) and immunofluorescence (IF/ICC) without permeabilization. However, appropriate controls should be included to confirm surface staining.
• For fixed-cell applications, a mild fixation protocol (e.g., 4% PFA) is recommended to preserve the fluorescence. If permeabilization is required for intracellular targets, verify that the epitope remains accessible.
• As a recombinant monoclonal antibody, this product offers batch-to-batch consistency. It is advisable to titrate the antibody in the relevant sample system to determine the optimal working concentration.
• TROP-2 expression can vary across cell lines and tissues; validate the antibody using a known positive control (e.g., a pancreatic carcinoma cell line) and a negative control (e.g., a TROP-2 knockout or knockdown system) for reliable results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. In the realm of tumor marker research, we offer validated monoclonal antibodies like anti-TROP-2, along with antibody pairs, bulk ancillary reagents, and assistance in sourcing rare targets. Our supply capabilities are designed to streamline your assay development, whether you need optimized clones for fluorescence-based applications or custom raw-material procurement.