Rabbit anti-Human TROP-2 Monoclonal Antibody for Flow Cytometry - P09758

Core Product Specifications and Parameters

Biological Background: TROP-2 Function and Localization

• TROP-2, encoded by the TACSTD2 gene, is also known as Cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1 (M1S1), and Pancreatic carcinoma marker protein GA733-1.
• The protein is predicted to act as a growth factor receptor, though its full biological function remains to be fully elucidated.
• TROP-2 is a single-pass type I membrane protein predominantly localized to the cell membrane.
• It exhibits high expression in placenta and pancreatic carcinoma cell lines, consistent with its role as a widely recognized tumor antigen.
• The protein structure includes features such as a disulfide bond and N-glycosylation sites, which contribute to its stability and cell surface expression.
• Mutations in TACSTD2 have been linked to corneal dystrophy and amyloidosis, underscoring its importance in both normal physiology and disease.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein spanning amino acids 27–274 of human TROP-2, which encompasses the extracellular domain; this design supports the detection of cell surface TROP-2 by flow cytometry in live-cell assays.
• Being a directly conjugated primary antibody (), it eliminates the need for secondary antibody incubation, reducing protocol time and potential background; validate the optimal concentration for your cell model.
• For flow cytometry, consider using standard staining protocols with FSC/SSC gating on viable cells, and include appropriate isotype controls to confirm specificity.
• This clone has been validated on human samples; cross-reactivity with other species has not been reported, so verify reactivity if using non-human systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio integrates a curated portfolio of IVD raw materials, including validated Trop-2 antibodies for tumor marker assay development. Beyond this monoclonal antibody, we offer antibody pairs, optimized conjugates, and bulk ancillary reagents to streamline your diagnostic workflow. Our team also provides rare-target raw-material sourcing and technical consulting, supporting your project from concept to clinic with reliable supply and professional guidance.