Rabbit anti-Human TREM1 Monoclonal Antibody for FC - Q9NP99

Core Product Specifications and Parameters

Biological Background: TREM1 Function and Localization

• TREM1 (Triggering receptor expressed on myeloid cells 1), also designated CD354 or TREM-1, is a cell surface receptor of the immunoglobulin superfamily that amplifies inflammatory responses upon ligand binding by forming a complex with TYROBP/DAP12. Related references: PMID:10799849, PMID:21393102
• Upon activation, TREM1 triggers a SYK-mediated tyrosine phosphorylation cascade, leading to downstream activation of BTK, MAPK1, MAPK3, and phospholipase C-gamma, which drives pro-inflammatory cytokine and chemokine release. Related references: PMID:14656437, PMID:21659545
• TREM1 amplifies inflammatory signals initiated by Toll-like receptor (TLR) and NOD-like receptor engagement, playing a key role in septic shock and atherosclerosis pathophysiology. Related references: PMID:11323674, PMID:21393102
• It also acts as a decoy receptor, counterbalancing its own pro-inflammatory activity through neutralization of its ligand.
• Subcellularly, TREM1 is located at the cell membrane and can be secreted.
• TREM1 is predominantly expressed in myeloid lineage cells—monocytes, macrophages, neutrophils, and dendritic cells—and is highly expressed in adult liver, lung, and spleen; its expression is associated with mature myeloid development. Related references: PMID:10799849, PMID:11922939
• TREM1 is a glycoprotein with intramolecular disulfide bonds, predicted to have a molecular weight of approximately 26 kDa; however, glycosylation and alternative splicing result in observed bands around 18–26 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 21-200 of the human TREM1 extracellular domain, suggesting the antibody recognizes the ligand-binding region.
• This -conjugated monoclonal antibody is validated for direct flow cytometry (FC) analysis of human TREM1.
• For FC, titrate the antibody to determine the optimal concentration using cells known to express TREM1, such as monocytes or macrophages.
• Consider co-staining with lineage markers to identify TREM1-positive myeloid subsets.
• Assess cell surface expression on live, unfixed cells to maintain optimal staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, offering validated antibody pairs, recombinant proteins, and bulk ancillary reagents to support diagnostic developers and researchers. For innate immunity and inflammation research targeting TREM1, we supply optimized monoclonal antibodies and can assist with custom raw-material sourcing to accelerate your assay development from concept to clinic.