Rabbit anti-Human TNFRSF10D/TRAILR4 Monoclonal Antibody for FC - Q9UBN6

Core Product Specifications and Parameters

Biological Background: TNFRSF10D/TRAILR4 Function and Localization

• TNFRSF10D, also known as decoy receptor 2 (DcR2), functions as a receptor for the cytotoxic ligand TRAIL but possesses a truncated death domain, rendering it incapable of transducing apoptotic signals; instead, it acts as a protective decoy by competing with functional death receptors (PubMed:9537512). Related references: PMID:9537512
• Its ability to induce the NF-kappa-B pathway is controversial: one report indicates it cannot (PubMed:9382840), while another suggests it can (PubMed:9430226). Related references: PMID:9382840 PMID:9430226
• The protein is a member of the tumor necrosis factor receptor superfamily, encoded by the TNFRSF10D gene (alias DCR2, TRAILR4, TRUNDD).
• It is localized to the cell membrane as a single-pass type I transmembrane protein.
• Key structural features include a signal peptide, three cysteine-rich TNFR/NGFR repeats, a transmembrane region, and a truncated death domain; post-translational modifications include glycosylation and disulfide bond formation.
• Widely expressed in many tissues, with higher levels in fetal kidney, lung, liver, and adult testis, liver; also found in peripheral blood leukocytes, colon, small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta, and heart.

Experimental Guidance and Technical Tips

• The immunogen (recombinant protein spanning extracellular amino acids 56-211) suggests the antibody may recognize the native receptor on live cells, making it a candidate for flow cytometry applications.
• As a directly conjugated antibody, it eliminates the need for a secondary detection step in FC, simplifying workflow and reducing background.
• For optimal performance in flow cytometry, consider titrating the antibody and validating staining in a relevant human cell line known to express TNFRSF10D; use an appropriate isotype control to assess non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

For researchers developing apoptosis-related diagnostics or studying TRAIL signaling, CamelBio offers comprehensive sourcing of validated antibody pairs, optimized monoclonal/polyclonal antibodies (like this anti-TNFRSF10D clone), and ancillary reagents. We also support rare target material procurement to accelerate your assay development from concept to clinic.