Rabbit anti-Human TLR1 Monoclonal Antibody for FC - Q15399

Core Product Specifications and Parameters

Biological Background: TLR1 Function and Localization

• TLR1 (Toll-like receptor 1), also known as TIL or CD281, is a member of the toll-like receptor family, which plays a fundamental role in pathogen recognition and activation of innate immunity.
• The receptor specifically recognizes diacylated and triacylated lipopeptides from bacteria and cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins. Related references: PMID:21078852
• Upon ligand binding, TLR1 forms an activation cluster with TLR2 and CD14 at the cell surface, triggering downstream signaling via MYD88 and TRAF6, leading to NF-kappa-B activation and pro-inflammatory cytokine secretion. Related references: PMID:16880211
• The TLR2:TLR1:CD14 complex is subsequently targeted to the Golgi apparatus in a lipid-raft dependent manner, highlighting dynamic trafficking upon activation.
• Subcellularly, TLR1 is located at the cell membrane, cytoplasmic vesicles, phagosome membrane, membrane rafts, and the Golgi apparatus, consistent with its role in pathogen sensing and signaling.
• TLR1 is ubiquitously expressed, with high levels in spleen, ovary, peripheral blood leukocytes, thymus, and small intestine.
• Key structural features include an extracellular domain with leucine-rich repeats (LRRs) responsible for ligand recognition, a single transmembrane helix, and a cytoplasmic TIR (Toll/interleukin-1 receptor) domain for signal transduction. It is a glycoprotein with disulfide bonds.

Experimental Guidance and Technical Tips

• The fluorophore is suitable for flow cytometry using a 488 nm laser; ensure detector settings are optimized for green fluorescence (e.g., FITC/GFP channel).
• The immunogen corresponds to amino acids 325–424 within the extracellular domain; this region is likely accessible for surface staining without permeabilization. However, cell fixation/permeabilization protocols should be tested if intracellular staining is desired.
• Use appropriate isotype controls and Fc receptor blocking to minimize non-specific binding in flow cytometry assays.
• Verify antibody performance in relevant biological samples (e.g., human peripheral blood mononuclear cells) and titrate for optimal staining index.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD and research organizations with streamlined access to critical raw materials, including validated antibody pairs, optimized monoclonal antibodies, conjugated reagents, and bulk ancillary supplies. For studies focused on innate immunity and toll-like receptors, we offer this anti-TLR1 mAb as part of a wider portfolio for immunology research and diagnostic development. Contact us to explore custom sourcing and technical support tailored to your project’s needs.