Rabbit anti-Human TIM-3/HAVCR2 mAb for Flow Cytometry - Q8TDQ0

Core Product Specifications and Parameters

Biological Background: TIM-3/HAVCR2 Function and Localization

• TIM-3 (HAVCR2) is a T-cell immunoglobulin and mucin domain-containing protein 3, also known as CD366, KIM-3, or TIMD-3. It is a cell surface receptor primarily associated with modulating innate and adaptive immune responses. Related references: PMID:11823861
• It generally exerts inhibitory functions, suppressing Th1-mediated auto- and alloimmune responses and promoting immunological tolerance. In CD8+ T cells, it attenuates TCR signaling by blocking NF-κB and NFAT activities, leading to reduced IL-2 secretion. Related references: PMID:14556005, PMID:24337741, PMID:26492563
• TIM-3 is expressed on various immune cells, including Th1 lymphocytes, Treg cells, dendritic cells, and natural killer cells. Its activity can be context-dependent, with some reports indicating stimulatory roles under certain conditions. Related references: PMID:24825777, PMID:24838857, PMID:22323453, PMID:22383801
• The protein localizes to the cell membrane and cell junctions. It contains an immunoglobulin domain, is glycosylated, palmitoylated, and undergoes phosphorylation and ubiquitination, as indicated by post-translational modification keywords.
• TIM-3 functions as a receptor for galectin-9 (LGALS9) and phosphatidylserine (PtSer). Binding to LGALS9 can lead to T-cell suppression or activation of innate responses against intracellular pathogens. Related references: PMID:16286920, PMID:24337741, PMID:23555261
• In dendritic cells, TIM-3 mediates engulfment of apoptotic cells and synergizes with Toll-like receptors to promote TNF secretion. In tumor-infiltrating DCs, it may suppress nucleic acid–mediated innate immune responses by interaction with HMGB1.
• TIM-3 expression is increased on CD4+ and CD8+ T cells in chronic HCV and HIV infections, highlighting its role in immune exhaustion. It is also expressed in epithelial tissues.
• The gene undergoes alternative splicing, and its protein product has a molecular weight of approximately 33.4 kDa.

Experimental Guidance and Technical Tips

• This antibody is generated against the extracellular domain (aa 22–200) of human TIM-3, providing specificity for the native conformation on live cells. Consider using it for surface staining in flow cytometry without permeabilization.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions. Include appropriate isotype controls and Fc receptor blocking steps.
• Although the antibody is validated for flow cytometry, cross-reactivity with other species has not been tested; its use is restricted to human samples. Validate specificity using TIM-3 overexpressing cells or knockdown/knockout models.
• TIM-3 is often co-expressed with other checkpoint molecules; consider multicolor panels to profile exhausted T cells in cancer or chronic infection models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers, labs, and research institutes, offering one-stop access to IVD raw materials and technical support from concept to clinic. This TIM-3/HAVCR2 antibody fits into immuno-oncology and immune profiling research, and we can source complementary validated antibody pairs, bulk ancillary reagents, and rare-target raw materials tailored to your assay development needs. Let CamelBio streamline your supply chain with high-quality antibodies and expert consultation.