Rabbit anti-Human TIGIT Monoclonal Antibody for Flow Cytometry - Q495A1

Core Product Specifications and Parameters

Biological Background: TIGIT Function and Localization

• TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9, VSTM3, or WUCAM, is an inhibitory receptor of the immunoglobulin superfamily.
• It suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Related references: PMID:19011627
• Upon binding to PVR/CD155 or NECTIN2/CD112 on antigen-presenting cells, TIGIT delivers inhibitory signals by recruiting Src kinases, GRB2, and SHIP1 to inhibit PI3K/MAPK pathways, and associates with beta-arrestin-2 to suppress NF-kappa-B signaling via TRAF6. Related references: PMID:23154388 PMID:24817116
• TIGIT also functions as a receptor for NECTIN4, inhibiting NK cell cytotoxicity. Related references: PMID:32503945
• It is localized at the cell membrane, consistent with its role in immune synapse modulation.
• TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells, and is upregulated following activation.
• Post-translational modifications include glycosylation and phosphorylation; the protein contains a disulfide bond and an ITIM domain.

Experimental Guidance and Technical Tips

• This -conjugated antibody is designed for direct flow cytometry; use appropriate isotype controls and optimize titration for your specific cell type and activation state.
• The immunogen corresponds to the extracellular region (aa 22-141), ensuring recognition of surface-expressed TIGIT on live cells.
• Since TIGIT is upregulated upon T-cell and NK cell activation, consider stimulating cells (e.g., with anti-CD3/CD28) prior to staining to enhance detection.
• For multicolor flow cytometry panels, verify compensation settings with single-stained controls and include viability dyes to exclude dead cells.
• Confirm the molecular weight band pattern (~18 kDa core, ~26 kDa glycosylated) if performing complementary western blot validation.

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CamelBio provides a one-stop sourcing bridge for IVD raw materials, including this high-quality anti-TIGIT monoclonal antibody for immune checkpoint research and diagnostic assay development. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Let CamelBio accelerate your project from concept to clinic with reliable supply and expert support.