Rabbit anti-Human T-bet/Tbx21 Monoclonal Antibody for Intracellular Flow Cytometry - Q9UL17

Core Product Specifications and Parameters

Biological Background: T-bet/Tbx21 Function and Localization

• T-bet (TBX21) is the master transcription factor for Th1 cell lineage commitment, initiating Th1 development by activating Th1-specific gene programs and simultaneously repressing Th2 and Th17 genetic programs. Related references: PMID:10761931

• It directly activates IFNG and CXCR3 gene expression by recruiting chromatin remodeling complexes, including KDM6B and SMARCA4-containing SWI/SNF, which establish a permissive chromatin topology. Related references: PMID:33296702

• T-bet recruits the super elongation complex (SEC) containing CDK9 and cyclin T1 to super-enhancers, promoting transcriptional elongation of Th1-associated genes. Related references: PMID:27292648

• It suppresses alternative T helper cell fates: it blocks Th17 differentiation by inhibiting RUNX1-mediated activation of RORC, and represses Th2 cytokines (IL-4, IL-5, IL-13) by downregulating GATA3 and NFATC2.

• T-bet protects Th1 cells from amplifying aberrant type-I interferon responses in IFN-gamma-rich environments by acting as a repressor of type-I IFN transcription factors and ISGs.

• It is required for the proper development of natural killer (NK) and mucosal-associated invariant T (MAIT) cells. Related references: PMID:33296702

• T-bet localizes to the nucleus and undergoes post-translational modifications including phosphorylation and ubiquitination, which regulate its activity.

Experimental Guidance and Technical Tips

• For intracellular staining of this nuclear target, ensure a robust fixation and permeabilization protocol optimized for transcription factors. Consider testing different permeabilization buffers to balance epitope accessibility and morphology preservation.
• Titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions; include an appropriate isotype control and fluorescence-minus-one (FMO) control to assess background.
• The immunogen corresponds to the C-terminal region (aa 322–535) of human T-bet, which may aid in detecting both full-length and potential splice variants, but cross-reactivity with other T-box family members should be verified in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing platform for IVD raw materials, supporting diagnostic manufacturers and research laboratories with validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents. This anti-T-bet antibody fits into Th1/Th2 immune profiling and infectious disease research workflows, and we can also source related reagents such as cytokine antibodies, isotype controls, and specialized staining buffers to streamline your assay development. Whether you need rare-target raw materials or large-scale supply continuity, CamelBio is your reliable partner from concept to clinic.