Rabbit anti-Human T-bet/Tbx21 Monoclonal Antibody for Flow Cytometry - Q9UL17

Core Product Specifications and Parameters

Biological Background: T-bet/Tbx21 Function and Localization

• T-bet (TBX21) is a T-box transcription factor, also known as T-cell-specific T-box transcription factor T-bet, T-PET, TBET, or TBLYM, and is the master regulator of Th1 lineage commitment.
• Drives Th1 differentiation by activating Th1 genetic programs (e.g., IFNG, CXCR3) and repressing Th2/Th17 programs; suppresses Th2 cytokines (IL-4, IL-5, IL-13) via repression of GATA3 and NFATC2, and inhibits Th17 by blocking RUNX1-mediated RORC transactivation. Related references: PMID:10761931
• Epigenetically remodels the IFNG locus by inducing permissive chromatin accessibility and CpG methylation, recruiting chromatin remodeling complexes including KDM6B, SWI/SNF, and an H3K4me2-methyltransferase complex. Related references: PMID:33296702
• Activates Th1 gene transcription also via recruitment of the Mediator complex and P-TEFb (super elongation complex) to super-enhancers in activated Th1 cells. Related references: PMID:27292648
• Protects Th1 cells from aberrant type-I IFN responses in an IFN-γ-rich microenvironment by acting as a repressor of type-I IFN transcription factors and IFN-stimulated genes.
• Required for the correct development of natural killer (NK) and mucosal-associated invariant T (MAIT) cells. Related references: PMID:33296702
• Localizes exclusively to the nucleus, consistent with its role as a transcription factor.
• Expression is T-cell specific; it is a key determinant in adaptive immunity and has implications in asthma (disease variant) and antiviral IgG2a class switching.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 322-535) of human T-bet; consider sequence conservation across species if cross-reactivity beyond human is desired, and validate in the relevant sample system.
• This antibody is validated for intracellular flow cytometry (FC intra). For optimal detection of nuclear T-bet, use fixation and permeabilization buffers optimized for nuclear antigens, and titrate the antibody to minimize background.
• Include appropriate isotype controls and compare resting vs. activated T-cell populations to confirm specificity, as T-bet expression is activation-dependent.
• For multiplex panels, note that can be combined with other fluorophores; ensure compensation controls are properly set.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with reliable IVD raw materials, offering one-stop access from concept to clinic. This anti-T-bet monoclonal antibody integrates seamlessly into Th1/Th2 immunophenotyping assays and immune profiling workflows. Beyond single antibodies, we supply validated antibody pairs, bulk custom conjugations, and rare-target raw material sourcing to accelerate your diagnostic development.