Rabbit anti-Human Syk Monoclonal Antibody for FC (intra) - P43405

Core Product Specifications and Parameters

Biological Background: Tyrosine-protein kinase SYK Function and Localization

• SYK (Spleen tyrosine kinase, p72-Syk) is a non-receptor tyrosine-protein kinase that mediates signal transduction downstream of diverse transmembrane receptors, including the B-cell receptor (BCR) essential for adaptive immunity (PubMed:12387735, PubMed:33782605). Related references: PMID:12387735, PMID:33782605
• Upon receptor engagement, SYK assembles into signaling complexes at the plasma membrane via its SH2 domains binding to phosphorylated ITAM motifs. It phosphorylates key effectors such as VAV1, PLCG1, and BLNK to propagate downstream cascades (PubMed:12456653, PubMed:15388330, PubMed:34634301). Related references: PMID:12456653, PMID:15388330, PMID:34634301
• SYK is critically required for B-cell maturation, particularly at the pro-B to pre-B transition, and also participates in T-cell receptor signaling (PubMed:12456653). Related references: PMID:12456653
• In innate immunity, SYK is activated by the membrane lectin CLEC7A upon fungal stimulation, leading to ROS production and NF-κB-mediated cytokine transcription. It is also required for IL15-induced neutrophil phagocytosis (PubMed:15123770). Related references: PMID:15123770
• SYK localizes to the cell membrane and cytoplasm/cytosol, enabling interaction with activated receptors and intracellular signaling molecules.
• Highly expressed in hematopoietic cells, including neutrophils, and present throughout B-cell development from pro-B to plasma cells (PubMed:8163536). Related references: PMID:8163536
• SYK serves non-immune functions, including osteoclast maturation, platelet activation, and vascular development (PubMed:12387735). Related references: PMID:12387735
• Keywords from UniProt highlight that SYK is an ATP-binding phosphoprotein with SH2 domains and tyrosine-protein kinase/transferase activity, classifying it as a key regulator of phosphorylation-dependent signaling.

Experimental Guidance and Technical Tips

• The immunogen comprises a synthetic peptide spanning residues 251–350 of human Syk (NP_003168.2), located within the kinase domain. This sequence is specific to human Syk; cross-reactivity with other species should be validated independently.
• For intracellular flow cytometry (FC intra), we recommend gentle fixation and permeabilization protocols optimized for the target cell type, as Syk may localize to both membrane-proximal and cytosolic compartments.
• The predicted molecular weight of full-length SYK is 72 kDa; a 70 kDa isoform has also been reported. Blot size should be verified in the target sample system.
• As a monoclonal antibody, it provides consistent specificity; perform titration to determine the optimal concentration for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials. This Rabbit anti-Human Syk mAb is part of our expanding portfolio of immune-signaling antibodies, complemented by validated antibody pairs, optimized clones for assay development, and bulk ancillary reagents. Our supply chain facilitates sourcing for rare and specialized targets, supporting your projects from concept to clinic.