Rabbit anti-Human Siglec-8 Monoclonal Antibody for Flow Cytometry - Q9NYZ4

Core Product Specifications and Parameters

Biological Background: Siglec-8 Function and Localization

• Serves as a putative adhesion molecule that mediates sialic-acid dependent binding to blood cells, with a preference for α-2,3-linked sialic acid. Related references: PMID:10625619 PMID:10856141
• Also binds α-2,6-linked sialic acid, though the binding site may be masked by cis interactions with sialic acids on the same cell. Related references: PMID:10625619
• Preferentially recognizes epitopes containing a terminal N-acetylneuraminic acid (Neu5Ac) and an underlying 6-O-sulfated galactose, such as the 6-sulfo sialyl Lewis X structure. Related references: PMID:27357658
• Localizes to the plasma membrane as a single-pass transmembrane protein.
• Exhibits restricted expression on peripheral blood cells, specifically basophils, mast cells, and eosinophils.
• Belongs to the immunoglobulin superfamily of Siglecs and contains characteristic Ig-like domains.
• Alternative splicing generates multiple transcript variants (UniProt keyword: Alternative splicing).

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular region (aa 17-363) containing sialic acid-binding domains, likely targeting native cell-surface epitopes accessible in flow cytometry.
• For flow cytometry, titrate the antibody to determine optimal staining concentration on human whole blood or isolated leukocyte populations known to express Siglec-8 (e.g., eosinophils, basophils).
• Include appropriate isotype controls and Fc receptor blocking to minimize non-specific binding.
• Siglec-8 may undergo cis interactions masking the binding site; consider cell activation or sialidase treatment to unmask epitopes if weak staining is observed.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material partner, CamelBio supplies this anti-Siglec-8 antibody alongside a wide portfolio of monoclonal and polyclonal antibodies, antigen proteins, and ancillary reagents to support immunoassay development and diagnostic research. Whether you require validated antibody pairs, bulk production, or rare-target sourcing, we streamline procurement from lead candidate to clinical application. Contact our team to discuss your Siglec-8 project needs.