Rabbit anti-Human Siglec-8 Monoclonal Antibody for Flow Cytometry - Q9NYZ4

Core Product Specifications and Parameters

Biological Background: Siglec-8 Function and Localization

• Siglec-8 (Sialic acid-binding Ig-like lectin 8), also known as SAF-2 (sialoadhesin family member 2), is a transmembrane receptor belonging to the Siglec family of sialic acid-binding lectins encoded by the SIGLEC8 gene (GeneID: 27181).
• Functions as a putative adhesion molecule mediating sialic-acid dependent binding to blood cells. Preferentially binds to α-2,3-linked sialic acid and, to a lesser extent, α-2,6-linked sialic acid. The binding site may be masked by cis interactions with sialic acids on the same cell surface. Related references: PMID:10625619 PMID:10856141
• Recognizes glycan epitopes containing a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose, with a strong preference for 6-O-sulfated sialyl-Lewis X structures. Related references: PMID:27357658
• The protein features multiple immunoglobulin-like domains, a transmembrane helix, and a cytoplasmic tail. Alternative splicing generates isoforms with predicted molecular weights of 44, 46, and 54 kDa, consistent with its mature glycosylated form.
• Localized to the plasma membrane, Siglec-8 is specifically expressed on blood cells, particularly basophils, mast cells, and eosinophils, making it a key marker for these lineages.
• Post-translational modifications include N-glycosylation and disulfide bond formation, essential for structural stability and ligand recognition. The three-dimensional structure has been experimentally determined (PDB available), highlighting its lectin domain architecture.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular sialic acid-binding domain (aa 17–363), so this antibody is likely to recognize native conformations – consider using it primarily for surface staining in flow cytometry.
• Validated for flow cytometry (FC) with human samples; for other applications (e.g., Western blot, IHC), in-house optimization including antigen retrieval and blocking conditions is advised.
• Due to restricted expression on eosinophils, mast cells, and basophils, include appropriate positive controls (e.g., purified eosinophils or cell lines) and isotype controls to confirm specificity.
• Titrate the antibody for optimal signal-to-noise ratio, as Siglec-8 expression levels may vary among cell types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and research labs with comprehensive IVD raw material solutions, covering every stage from concept to clinic. For Siglec-8-related projects, we supply validated monoclonal antibodies like this conjugate for flow cytometry, along with matched antibody pairs, recombinant proteins, and ancillary reagents. Whether you need bulk quantities, custom conjugation, or assistance with rare target sourcing, our team ensures reliable supply and technical support to accelerate your assay development.