Rabbit anti-Human Siglec-8 Monoclonal Antibody for Flow Cytometry - Q9NYZ4

Core Product Specifications and Parameters

Biological Background: Siglec-8 Function and Localization

• Siglec-8 is a sialic acid-binding Ig-like lectin, also known as sialoadhesin family member 2 (SAF-2). It is encoded by the SIGLEC8 gene and is a member of the CD33-related Siglec subfamily.
• Functions as a putative adhesion molecule that mediates sialic-acid dependent binding to blood cells, preferentially recognizing α2,3-linked sialic acid and also binding to α2,6-linked sialic acid. Related references: PMID:10625619 PMID:10856141
• Recognizes epitopes requiring a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose, with a preference for Gal-6-sulfated sialyl-Lewis X glycans. Related references: PMID:27357658
• The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface, modulating its binding activity.
• Expressed specifically on the surface of eosinophils, basophils, and mast cells, indicating its role in allergic and inflammatory responses.
• Subcellularly localized to the membrane, consistent with its function as a transmembrane receptor.
• Contains an N-terminal signal peptide, extracellular Ig-like domains (Ig-like V-type and C2-type), a transmembrane helix, and a cytoplasmic tail; classified as a glycoprotein with disulfide bonds.
• Multiple protein isoforms exist due to alternative splicing, with molecular weights of 44kDa, 46kDa, and 54kDa reported.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 17–363, which covers the extracellular region, likely ensuring recognition of native surface-expressed Siglec-8.
• This antibody is validated for flow cytometry (FC); consider titrating from 0.1–1 μg per test to determine optimal staining concentration for your cell population.
• For human whole blood or PBMC staining, include Fc receptor blocking step and appropriate isotype controls to minimize non‑specific binding.
• Double‑check specificity by comparing staining on Siglec-8‑positive primary cells (eosinophils, basophils) versus Siglec-8‑negative lymphocytes.
• Due to potential cis‑masking of the epitope, verify that sialidase treatment of cells does not alter antibody binding if needed for your assay.
• Use a viable cell gate and, if fixing, ensure that fixation/permeabilization conditions do not distort the epitope recognized by the antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to specialized IVD raw materials, including validated monoclonal antibodies like this anti‑Siglec‑8 reagent for immune cell profiling. Our portfolio spans optimized antibody pairs, bulk ancillary reagents, and custom sourcing of rare‑target raw materials to accelerate your assay development. For partners investigating allergic inflammation or hematological disorders, CamelBio offers comprehensive supply solutions—from concept to clinical application.