Rabbit anti-Human Siglec-8 Monoclonal Antibody for FC - Q9NYZ4

Core Product Specifications and Parameters

Biological Background: Siglec-8 Function and Localization

• Siglec-8 (sialic acid-binding Ig-like lectin 8), also designated sialoadhesin family member 2 (SAF-2), is a member of the sialoadhesin family of lectins.
• It is a putative adhesion molecule that mediates sialic-acid dependent binding to blood cells. Related references: PMID:10625619 PMID:10856141
• Siglec-8 preferentially binds to α-2,3-linked sialic acid and, to a lesser extent, α-2,6-linked sialic acid; cis interactions on the same cell surface may mask the binding site. Related references: PMID:10625619
• It recognizes terminal N-acetylneuraminic acid (sialic acid) with an underlying 6-O-sulfated galactose, displaying a preference for Gal-6-sulfated sialyl-Lewis X glycan epitopes. Related references: PMID:27357658
• The protein is a single-pass type I membrane glycoprotein containing immunoglobulin-like domains, a signal peptide, and a transmembrane helix; it features disulfide bonds and potential glycosylation sites.
• Expression is highly restricted to blood cells, primarily basophils, mast cells, and eosinophils.
• Alternative splicing generates isoforms with predicted molecular weights of 44 kDa, 46 kDa, and 54 kDa.

Experimental Guidance and Technical Tips

• The immunogen (extracellular domain, aa 17–363) suggests the antibody recognizes the membrane-proximal region of human Siglec-8; consider verifying cell surface staining versus intracellular staining in your assay.
• Since this is a directly conjugated antibody, no secondary staining step is required; ensure minimal light exposure and fix cells if needed for flow cytometry.
• Titrate the antibody to determine the optimal concentration for your specific sample type; typical starting dilutions range from 1:50 to 1:200, but this should be empirically validated.
• Use appropriate isotype control conjugated to the same fluorophore to assess background binding.
• Siglec-8 expression is restricted to basophils, mast cells, and eosinophils—co-stain with lineage markers (e.g., CD45, CD3, CD19, CD16, CD123, HLA-DR) to confirm cell populations.
• For whole blood staining, consider red blood cell lysis or density gradient separation to enrich leukocytes, and validate gating strategies with known positive and negative controls.

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CamelBio provides comprehensive access to IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents for diagnostic development and research. With deep expertise in immunology-related targets like Siglec-8, CamelBio supports manufacturers and laboratories from concept to clinic with reliable bulk sourcing, custom technical consulting, and seamless supply chain solutions. Let CamelBio be your procurement bridge for high-quality raw materials in allergy, asthma, and eosinophilic disorder studies.