Rabbit anti-Human ROR1 Monoclonal Antibody for Flow Cytometry - Q01973

Core Product Specifications and Parameters

Biological Background: ROR1 Function and Localization

• ROR1 is an inactive tyrosine-protein kinase transmembrane receptor, encoded by the ROR1 gene (also known as NTRKR1). It belongs to the receptor tyrosine kinase family and features immunoglobulin-like and kringle domains.
• Alternative splicing produces a short isoform that is highly expressed in fetal and adult central nervous system (CNS) and various cancers, whereas the full-length isoform is expressed in heart, lung, and kidney.
• ROR1 has very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo. Related references: PMID:25029443
• It acts as a receptor for WNT5A, activating the NFkB signaling pathway and potentially inhibiting WNT3A-mediated signaling. Related references: PMID:25029443 PMID:27162350
• In the inner ear, ROR1 is crucial for spiral ganglion neurons to innervate auditory hair cells; mutations in ROR1 are associated with non-syndromic deafness. Related references: PMID:27162350
• Through its ligand IGFBP5, ROR1 forms a complex with ERBB2 to enhance CREB oncogenic signaling, highlighting its role in cancer. Related references: PMID:36949068
• Subcellularly, ROR1 localizes to the cell membrane and is found on axonal projections, consistent with its role in cell signaling and neuronal development.
• Post-translational modifications include N-linked glycosylation, and the protein contains disulfide bonds critical for its domain architecture.

Experimental Guidance and Technical Tips

• This antibody was raised against the extracellular domain (amino acids 24-403), making it suitable for detecting cell surface ROR1 in live-cell flow cytometry.
• It is directly conjugated to , a bright and photostable fluorophore, simplifying experimental workflows by eliminating secondary staining.
• For FC, titrate the antibody to determine the optimal concentration, and include appropriate isotype controls and viability dyes for accurate gating.
• Validation is recommended in relevant human cell lines or primary samples known to express ROR1, such as certain cancer cell lines or neuronal cells.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material provider, CamelBio offers a one-stop supply solution for antibodies and ancillary reagents, supporting cancer and neurobiology research areas like ROR1-targeted studies. Our portfolio includes validated monoclonal antibodies, conjugated antibodies for flow cytometry, and recombinant proteins, enabling streamlined assay development from concept to clinic. With comprehensive sourcing capabilities, we assist diagnostic manufacturers and labs in obtaining rare target reagents, ensuring project continuity and rapid turnaround.