Rabbit anti-Human ROR1 Monoclonal Antibody for Flow Cytometry - Q01973

Core Product Specifications and Parameters

Biological Background: ROR1 Function and Localization

• ROR1, also known as Neurotrophic tyrosine kinase receptor-related 1 (NTRKR1), is an inactive tyrosine-protein kinase transmembrane receptor that serves as a receptor for WNT5A. It has very low kinase activity and is unlikely to function as a tyrosine kinase in vivo. Related references: PMID:25029443
• Binding of WNT5A to ROR1 activates the NFkB signaling pathway and may inhibit WNT3A-mediated signaling, implicating it in non-canonical Wnt pathways. Related references: PMID:25029443, PMID:27162350
• In the inner ear, ROR1 is crucial for spiral ganglion neurons to innervate auditory hair cells, highlighting its developmental role. Related references: PMID:27162350
• Through interaction with IGFBP5, ROR1 forms a complex with ERBB2 to enhance CREB oncogenic signaling, contributing to cancer progression. Related references: PMID:36949068
• ROR1 is localized to the cell membrane and cell projections such as axons, consistent with its role in cell surface signaling.
• Alternative splicing produces a short isoform that is strongly expressed in fetal and adult CNS and in various human cancers, while the full-length protein is prominently expressed in heart, lung, and kidney.
• ROR1 contains immunoglobulin-like and kringle domains, and is modified by glycosylation and disulfide bonds, which may influence its stability and ligand interactions.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 24-403) encompasses the extracellular region of human ROR1; consider this design when planning epitope competition or domain-specific studies.
• This antibody is validated for flow cytometry (FC). Optimize the antibody concentration by titration on appropriate positive and negative control cells to achieve optimal staining.
• Because ROR1 is a cell surface receptor, the conjugate may be suitable for live-cell staining without permeabilization; however, confirm in your specific experimental system.
• For other applications or species cross-reactivity, validate the antibody under your own conditions, as the provided data only cover human reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this validated anti-ROR1 monoclonal antibody, to support diagnostic manufacturers and researchers focusing on oncology, developmental biology, and Wnt signaling. We provide bulk quantities, custom conjugation services, and access to rare target antibodies, ensuring a reliable supply chain for your immunoassay and research reagent needs from concept to clinic.