Rabbit anti-Human PTH1R Monoclonal Antibody () for Flow Cytometry - Q03431

Core Product Specifications and Parameters

Biological Background: PTH1R Function and Localization

• PTH1R is a G protein-coupled receptor that binds parathyroid hormone (PTH) and PTH-related peptide (PTHLH), initiating intracellular signaling cascades (PubMed:10913300, 18375760, 19674967). Related references: PMID:10913300 PMID:18375760 PMID:19674967
• It couples to G(s) alpha proteins to activate adenylate cyclase, leading to increased cAMP levels (PubMed:20172855, 30975883). Related references: PMID:20172855 PMID:30975883
• PTHLH dissociates from PTH1R more rapidly than PTH, causing a faster decay of the cAMP response (PubMed:35932760). Related references: PMID:35932760
• The receptor is located at the cell membrane.
• Expressed in most tissues, with highest levels in kidney, bone, and liver.
• Contains key structural features including disulfide bonds, glycosylation sites, and phosphorylation sites; associated with diseases such as dwarfism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 27-188) of human PTH1R, which is accessible on the cell surface without permeabilization. This design supports detection in live-cell flow cytometry.
• For flow cytometry, consider titrating the -conjugated antibody to determine the optimal staining dilution (e.g., 1:50 – 1:200) using cells known to express PTH1R, such as kidney or bone cell lines.
• Use appropriate negative controls (e.g., isotype control or cells with low/absent PTH1R expression) to set gates and assess specificity.
• The large Stokes shift of minimizes spectral overlap; verify instrument compatibility (excitation ~650 nm, emission ~665 nm).
• Fixation with aldehydes may alter the epitope; if fixation is required, test whether it affects antibody binding.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio is a trusted one-stop partner for IVD raw-material procurement, offering a full spectrum of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing solutions for rare targets.
• This anti-PTH1R antibody, targeting the parathyroid hormone receptor, supports research and diagnostic development in endocrinology, bone metabolism, and calcium homeostasis disorders.
• From early concept through clinical application, CamelBio bridges your sourcing gaps with reliable quality and expert technical support.