Rabbit anti-Human PTH1R Monoclonal Antibody for Flow Cytometry - Q03431

Core Product Specifications and Parameters

Biological Background: PTH1R Function and Localization

• PTH1R serves as a receptor for both parathyroid hormone (PTH) and parathyroid hormone-related peptide (PTHLH), initiating intracellular signaling upon ligand binding. Related references: PMID:10913300, PMID:18375760, PMID:19674967

• Coupling primarily to G(s) alpha subunits, PTH1R activates adenylate cyclase, leading to increased cAMP production. Related references: PMID:20172855, PMID:30975883, PMID:35932760

• PTHLH exhibits faster dissociation from PTH1R than PTH, resulting in a more transient cAMP response. Related references: PMID:35932760

• The receptor localizes to the cell membrane, consistent with its role as a transmembrane signal transducer.

• PTH1R is widely expressed, with highest levels in kidney, bone, and liver.

• Contains structural features typical of GPCRs, including transmembrane helices, disulfide bonds, and glycosylation sites.

• Mutations in PTH1R are associated with skeletal dysplasias, such as chondrodysplasia.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal extracellular domain (residues 27-188), accessible for live-cell flow cytometry. Consider appropriate cell surface staining protocols.

• For FC, titrate the antibody to determine the optimal concentration using PTH1R-expressing cell lines or primary cells. Include isotype and unstained controls.

• direct conjugate eliminates the need for secondary detection, reducing background and experimental time.

• Verify PTH1R expression in your sample system by complementary methods (e.g., qPCR or western blot) to correlate with flow cytometry results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and premium monoclonal/polyclonal antibodies like this anti-PTH1R clone. We can source bulk ancillary reagents and assist with custom projects targeting GPCRs and signaling pathways. For your bone and kidney research applications, CamelBio offers reliable supply solutions, streamlining your journey from concept to clinic.