Rabbit anti-Human Podoplanin Monoclonal Antibody for Flow Cytometry - Q86YL7

Core Product Specifications and Parameters

Biological Background: Podoplanin Function and Localization

• Podoplanin (PDPN), also known as Aggrus, Gp36, or T1-alpha, is a small, extensively O-glycosylated transmembrane glycoprotein with multiple isoforms generated by alternative splicing.
• It mediates cell migration and adhesion through interactions with partners such as CLEC1B, CD9, CD44, MSN, EZR, and LGALS8, playing key roles in platelet aggregation, epithelial-mesenchymal transition (EMT), and tumor invasiveness. Related references: PMID:14522983 PMID:15231832 PMID:17222411 PMID:18541721 PMID:17046996 PMID:21376833 PMID:20962267 PMID:19268462
• During development, PDPN is essential for proper separation of blood and lymphatic vessels by triggering CLEC1B-dependent platelet activation. Related references: PMID:14522983 PMID:15231832 PMID:17222411
• In tumor cells, PDPN stabilizes invadopodia and promotes extracellular matrix degradation via the RHOC–ROCK–LIMK–CFL1 pathway, enhancing invasiveness. Related references: PMID:25486435
• PDPN localizes predominantly to the plasma membrane and is enriched in membrane protrusions such as lamellipodia, filopodia, microvilli, ruffles, and invadopodia; it also resides in membrane rafts and cytosol.
• Tissue expression is high in placenta, lung, skeletal muscle, and brain, with upregulation observed in colorectal tumors and a subset of oral squamous cell carcinomas.
• Post-translational modifications include extensive O-glycosylation and sialic acid addition, influencing protein structure and interactions.
• Despite structural homology, PDPN does not function as a water channel or regulate aquaporins. Related references: PMID:9651190

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal extracellular region (amino acids 21-123), making this conjugate well-suited for detecting surface-expressed Podoplanin by flow cytometry without permeabilization.
• For flow cytometry, validate the antibody in a relevant human cell system known to express PDPN, such as lymphatic endothelial cells, placental tissue, or tumor cell lines (e.g., colorectal or oral squamous carcinoma).
• Consider that PDPN exhibits multiple isoforms and glycosylation variants; band patterns in western blot may differ from predicted molecular weights, though this conjugate is primarily intended for FC.
• Optimization of antibody titration and appropriate isotype controls is recommended for reliable flow cytometric analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers by providing integrated access to high-quality IVD raw materials like this anti-Podoplanin antibody, supporting cancer biomarker research and developmental studies. As a comprehensive sourcing partner, we offer validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and customized procurement for rare targets—streamlining your transition from concept to clinic with reliable supply chain solutions.