Rabbit anti-Human Podoplanin Monoclonal Antibody for FC - Q86YL7

Core Product Specifications and Parameters

Biological Background: Podoplanin Function and Localization

• Podoplanin (PDPN), also known as Aggrus, Gp36, or T1-alpha, is a type I transmembrane glycoprotein with multiple isoforms generated by alternative splicing.
• It mediates cell migration and adhesion by interacting with various partners: binding to CLEC1B triggers platelet activation/aggregation, while CD9 attenuates this response. Related references: PMID:14522983, PMID:15231832, PMID:17222411, PMID:18541721
• PDPN promotes epithelial-mesenchymal transition (EMT) via MSN or EZR, leading to RHOA activation and increased cell migration and invasiveness. Related references: PMID:17046996, PMID:21376833
• Interaction with CD44 drives directional cell migration in epithelial and tumor cells, while binding to LGALS8 may link the lymphatic endothelium to the extracellular matrix. Related references: PMID:20962267, PMID:19268462
• In tumor cells, PDPN controls invadopodia stability and extracellular matrix degradation via RHOC-ROCK-LIMK-CFL1 signaling. Related references: PMID:25486435
• Subcellular localization includes membrane raft, lamellipodium, filopodium, microvillus, ruffle, invadopodium, and apical/basolateral membranes; also detected in the cytosol.
• Highly expressed in placenta, lung, skeletal muscle, and brain; weakly in kidney and liver. Up-regulated in colorectal tumors and a subset of oral squamous cell carcinomas.
• Heavily glycosylated with sialic acid modifications, contributing to the observed multiple molecular weight forms (12–24 kDa).

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 21–123), making this antibody suitable for detection of surface Podoplanin by flow cytometry.
• For FC, titrate the antibody to determine the optimal working dilution using a known Podoplanin-expressing human cell line (e.g., lymphatic endothelial cells or tumor cell lines).
• Include appropriate isotype controls and viability dyes to ensure specific staining and exclude dead cells.
• Given the multiple glycosylation forms, confirm expression patterns in the relevant cell system; the observed signal intensity may vary with glycosylation state.
• This antibody is validated for human reactivity; cross-reactivity with other species is not expected.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research laboratories, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom raw-material procurement. This anti-Podoplanin monoclonal antibody supports research in cell migration, cancer biology, and lymphatic development. Contact us to explore how our one-stop sourcing capabilities can streamline your diagnostic and research workflows.